Inactivation of Ebola virus with a surfactant nanoemulsion

Hemorrhagic fever caused by Ebola virus (EBO) is a highly contagious infection. This necessitates that the contaminated instruments, clothes, and hospital premises must be completely disinfected. Nanoemulsions are a new form of disinfectant composed of detergents and vegetable oil suspended in water...

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Bibliographic Details
Published in:Acta tropica 2003-08, Vol.87 (3), p.315-320
Main Authors: Chepurnov, A.A, Bakulina, L.F, Dadaeva, A.A, Ustinova, E.N, Chepurnova, T.S, Baker, J.R
Format: Article
Language:English
Subjects:
Animals
Cercopithecus aethiops
Disinfection
Disinfection - methods
Ebola virus
Ebolavirus - drug effects
Ebolavirus - growth & development
Emulsions
Hemorrhagic Fever, Ebola - prevention & control
Nanoemulsion
Plant Oils
Surface-Active Agents
Vero Cells
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Summary:Hemorrhagic fever caused by Ebola virus (EBO) is a highly contagious infection. This necessitates that the contaminated instruments, clothes, and hospital premises must be completely disinfected. Nanoemulsions are a new form of disinfectant composed of detergents and vegetable oil suspended in water. The antiviral activity of nanoemulsion ATB has been investigated against EBO. The nanoemulsion was tested against two preparations of EBO (strain Zaire) obtained from Vero cell culture fluid (EBO-zc) and from blood of infected monkeys (EBO-zb). The nanoemulsion ATB was virucidal against both preparations of EBO, inactivating the purified virus within 20 min even when diluted 1:100 with the growth medium. Inactivation of the virus in tissue preparations was also complete, but required 1:10 dilutions with media or higher. After treatment with ATB (10 and 1% concentrations), no EBO was apparent even after two passages in Vero cell culture. These data indicate that the nanoemulsion is an effective disinfectant for EBO. Because of the excellent biocompatibility of nanoemulsions, studies are planned to determine whether the nanoemulsion-killed virus is suitable for developing a vaccine against EBO.
ISSN:0001-706X
1873-6254
DOI:10.1016/S0001-706X(03)00120-7