Patterns of responsiveness of T cell lines and thymocytes reveal waves of specific activity in the post-natal murine thymus

Antibody binding of CD3, CD4, or CD8 molecules can induce cytoplasmic calcium mobilization in T lymphocytes, usually Interpreted as indicating signal transduction. Using such assays, in a CD4+CD8+ thymocyte line and its single positive progeny we have Identified characteristic patterns of responsive...

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Bibliographic Details
Published in:International immunology 1992-10, Vol.4 (10), p.1091-1101
Main Authors: Morin, Cécile, Jotereau, Francine, Augustin, Andrei
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn - immunology
Biological and medical sciences
Calcium - metabolism
calcium mobilization
CD3 Complex - analysis
CD4 Antigens - analysis
CD8 Antigens - analysis
Cell Line, Transformed
Cell Movement
Clone Cells - immunology
cross-linking
F(ab) fragments
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Hematopoietic Stem Cells - cytology
Immunobiology
Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation
Mice
Mice, Inbred BALB C - immunology
Moloney murine leukemia virus
Signal Transduction
stimulation Index
T-Lymphocyte Subsets - immunology
Thy 1+ lymphocytes
thymocytes
Thymoma - immunology
Thymoma - microbiology
Thymoma - pathology
Thymus Gland - growth & development
Thymus Gland - immunology
Thymus Gland - pathology
Thymus Neoplasms - immunology
Thymus Neoplasms - microbiology
Thymus Neoplasms - pathology
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Summary:Antibody binding of CD3, CD4, or CD8 molecules can induce cytoplasmic calcium mobilization in T lymphocytes, usually Interpreted as indicating signal transduction. Using such assays, in a CD4+CD8+ thymocyte line and its single positive progeny we have Identified characteristic patterns of responsiveness that are reproducible in vivo in a subpopulatlon of newborn ‘double positive’ thymocytes but virtually absent in adult thymuses. In particular, these cells appear to be high responders to the binding of anti-CD3 F(ab)'2 fragments. We have followed the presence of such highly responsive thymocytes in the perinatal period and the first 15 days of life. Intriguingly, these cells populate the newborn thymus in three distinct waves. Such patterns of responsiveness may define early ’selectable‘ thymocytes.
ISSN:0953-8178
1460-2377
DOI:10.1093/intimm/4.10.1091