The Polypeptide Structure of Canine Coronavirus and its Relationship to Porcine Transmissible Gastroenteritis Virus

Department of Microbiology Agricultural Research Council Institute for Research on Animal Diseases, Compton, Newbury, Berkshire RG16 0NN, U.K. Canine coronavirus (CCV) isolate 1–71 was grown in secondary dog kidney cells and purified by rate zonal centrifugation. Polyacrylamide gel electrophoresis r...

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Bibliographic Details
Published in:Journal of general virology 1981-01, Vol.52 (1), p.153-157
Main Authors: Garwes, D. J, Reynolds, D. J
Format: Article
Language:English
Subjects:
Animals
Coronaviridae - analysis
Dogs - microbiology
Glycopeptides - analysis
Molecular Weight
Peptides - analysis
Transmissible gastroenteritis virus - analysis
Viral Proteins - analysis
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Summary:Department of Microbiology Agricultural Research Council Institute for Research on Animal Diseases, Compton, Newbury, Berkshire RG16 0NN, U.K. Canine coronavirus (CCV) isolate 1–71 was grown in secondary dog kidney cells and purified by rate zonal centrifugation. Polyacrylamide gel electrophoresis revealed four major structural polypeptides with apparent mol. wt. of 203800 (gp204), 49800 (p50), 31800 (gp32) and 21600 (gp22). Incorporation of 3 H-glucosamine into gp204, gp32 and gp22 indicated that these were glycopolypeptides. Comparison of the structural polypeptides of CCV and porcine transmissible gastroenteritis virus (TGEV) by co-electrophoresis demonstrated that TGEV polypeptides corresponded closely, but not identically, with gp204, p50 and gp32 of CCV and confirmed that gp22 was a major structural component only in the canine virus. The close similarities in structure of the two coronaviruses augments the relationship established by serology. Received 16 May 1980; accepted 21 July 1980.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-52-1-153