The Streptomyces coelicolor ssgB gene is required for early stages of sporulation

ssgB was identified as a novel early sporulation gene in Streptomyces coelicolor. An ssgB deletion mutant failed to sporulate, over-produced actinorhodin, and its colonies were significantly larger than those of the parental strain, suggesting an important role for the ssgB gene product in the proce...

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Bibliographic Details
Published in:FEMS microbiology letters 2003-08, Vol.225 (1), p.59-67
Main Authors: Keijser, Bart J.F, Noens, Elke E.E, Kraal, Barend, Koerten, Henk K, van Wezel, Gilles P
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
Bacterial Proteins - physiology
Bacteriology
Biological and medical sciences
Cell division
Development
Fundamental and applied biological sciences. Psychology
Gene Deletion
Gene disruption
Genes, Bacterial
Genetic Complementation Test
Genetics
Growth, nutrition, cell differenciation
Microbiology
Microscopy, Electron
Microscopy, Electron, Scanning
Phenotype
Sodium Chloride
Spores, Bacterial - genetics
Spores, Bacterial - physiology
Spores, Bacterial - ultrastructure
Sporulation
SsgA
Streptomyces
Streptomyces - genetics
Streptomyces - physiology
Streptomyces - ultrastructure
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Summary:ssgB was identified as a novel early sporulation gene in Streptomyces coelicolor. An ssgB deletion mutant failed to sporulate, over-produced actinorhodin, and its colonies were significantly larger than those of the parental strain, suggesting an important role for the ssgB gene product in the process of growth cessation prior to sporulation-specific cell division. This places ssgB temporally before the paralogous sporulation gene ssgA. Analysis of ssgB mutant hyphae by electron microscopy and by confocal fluorescence microscopy showed that it was defective in the initiation of sporulation, as no sporulation septa could be identified, and DNA segregation had not yet been initiated in the mutant.
ISSN:0378-1097
1574-6968
DOI:10.1016/S0378-1097(03)00481-6