Comparison between Rules-Based Human Immunodeficiency Virus Type 1 Genotype Interpretations and Real or Virtual Phenotype: Concordance Analysis and Correlation with Clinical Outcome in Heavily Treated Patients

• Published in: The Journal of infectious diseases 2003-07, Vol.188 (2), p.194-201
• Main Authors: Torti, Carlo, Quiros-Roldan, Eugenia, Keulen, Wilco, Scudeller, Luigia, Lo Caputo, Sergio, Boucher, Charles, Castelli, Francesco, Mazzotta, Francesco, Pierotti, Piera, Been-Tiktak, Anne Mieke, Buccoliero, Giovanni, De Gennaro, Michele, Carosi, Giampiero, Tinelli, Carmine
• Format: Article
• Language: English
• Subjects: Adult; AIDS; amprenavir; Anti-HIV Agents - pharmacology; Anti-HIV Agents - therapeutic use; Antiretrovirals; Antivirals; Biological and medical sciences; Drug resistance; Drug Resistance, Viral; Female; Genes, Viral - genetics; Genotype; Genotypes; Health outcomes; HIV; HIV 1; HIV Infections - drug therapy; HIV Infections - virology; HIV-1 - drug effects; HIV-1 - genetics; HIV/AIDS; Human immunodeficiency virus 1; Human viral diseases; Humans; Infectious diseases; Male; Medical sciences; Odds Ratio; Phenotype; Phenotypes; Risk Factors; Salvage Therapy; Treatment Outcome; Viral diseases; Viral diseases of the lymphoid tissue and the blood. Aids; Virology
• ISSN: 0022-1899; 1537-6613
• DOI: 10.1086/376512

We compared 2 rules-based genotype interpretation systems and real or virtual phenotype through a retrospective analysis of a prospective trial. Genotypes were determined with VircoGEN II (VIRCO) and were interpreted with either RetroGram 1.4 or TRUGENE HIV-1 (guidelines 3.0) or original virtual phenotype (Virtual Phenotype; VIRCO), as available in the year 2000. Among 188 human immunodeficiency virus (HIV) type 1 isolates, overall concordance (κ agreement) was observed for the 2 rules-based systems, whereas striking discordances were noted between them and real and virtual phenotype interpretations for stavudine, didanosine, zalcitabine, abacavir, and amprenavir (κ