Coexistent naïve phenotype and higher cycling rate of cord blood T cells as compared to adult peripheral blood

Umbilical cord blood (UCB) T cells are predominantly CD45RA(+), secrete less cytokines, and have diminished cytotoxicity compared to adult peripheral blood (PB). We hypothesized that the functional impairment of bulk UCB cells results from the relative dominance of immature lymphocyte subsets. In th...

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Bibliographic Details
Published in:Experimental hematology 2003-08, Vol.31 (8), p.708-714
Main Authors: Szabolcs, Paul, Park, Kyung-Duk, Reese, Melissa, Marti, Luciana, Broadwater, Gloria, Kurtzberg, Joanne
Format: Article
Language:English
Subjects:
Adult
Aging - blood
Antigens, CD - analysis
Cell Cycle
Fetal Blood - cytology
Humans
Immunophenotyping
Lymphocyte Count
T-Lymphocyte Subsets - cytology
T-Lymphocyte Subsets - immunology
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Summary:Umbilical cord blood (UCB) T cells are predominantly CD45RA(+), secrete less cytokines, and have diminished cytotoxicity compared to adult peripheral blood (PB). We hypothesized that the functional impairment of bulk UCB cells results from the relative dominance of immature lymphocyte subsets. In this study we established the physiologic ranges of lymphocyte subsets in UCB, and contrasted those with adult PB. Four-color FACS was utilized to characterize surface and intracellular protein expression on lymphocyte subsets from fresh unmanipulated UCB and adult PB. We found that UCB contain significantly higher absolute numbers of T cells, NK cells, and B cells than adult PB (p
ISSN:0301-472X
DOI:10.1016/S0301-472X(03)00160-7