Tissue- and stressor-specific differential expression of two hsc70 genes in carp

Two genes expressing 70 kDa heat shock proteins were identified in Cyprinus carpio. The sequence similarities and the intron-interrupted structure of the coding regions indicate that carp Hsc70-1 and Hsc70-2 belong to the Hsp70 cognate subfamily. The expressions of the two hsc70 genes were followed...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2003-08, Vol.307 (3), p.503-509, Article 503
Main Authors: Ali, Khaled Said, Dorgai, László, Ábrahám, Magdolna, Hermesz, Edit
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Cadmium - pharmacology
Carp
Carps - metabolism
Cd treatment
Cold shock
Cyprinus carpio
Differential expression
Gene Expression Regulation
Hsc70
hsc70 gene
HSC70 Heat-Shock Proteins
HSP70 Heat-Shock Proteins - biosynthesis
HSP70 Heat-Shock Proteins - classification
HSP70 Heat-Shock Proteins - genetics
Liver - metabolism
Molecular Sequence Data
Muscles - metabolism
Organ Specificity
Phylogeny
Protein Isoforms - biosynthesis
Protein Isoforms - genetics
Sequence Alignment
Teleostei
Temperature
Tissue Distribution
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Summary:Two genes expressing 70 kDa heat shock proteins were identified in Cyprinus carpio. The sequence similarities and the intron-interrupted structure of the coding regions indicate that carp Hsc70-1 and Hsc70-2 belong to the Hsp70 cognate subfamily. The expressions of the two hsc70 genes were followed by semi-quantitative RT-PCR. Both genes are expressed under unstressed conditions in a characteristic tissue-specific manner. Inducibility of the response to elevated temperature, cold shock, and Cd treatment was investigated in the liver and muscle, in whole-animal experiments. Both genes were insensitive to or only weakly induced by the stressors, with two exceptions: Cd treatment resulted in an 11–13-fold enhanced induction of hsc70-1 in the liver and cold shock enhanced induction of hsc70-2 in the muscle by 7.5–10-fold.
ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(03)01206-3