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Tissue- and stressor-specific differential expression of two hsc70 genes in carp
Two genes expressing 70 kDa heat shock proteins were identified in Cyprinus carpio. The sequence similarities and the intron-interrupted structure of the coding regions indicate that carp Hsc70-1 and Hsc70-2 belong to the Hsp70 cognate subfamily. The expressions of the two hsc70 genes were followed...
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| Published in: | Biochemical and biophysical research communications 2003-08, Vol.307 (3), p.503-509, Article 503 |
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| container_start_page | 503 |
| container_title | Biochemical and biophysical research communications |
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| creator | Ali, Khaled Said Dorgai, László Ábrahám, Magdolna Hermesz, Edit |
| description | Two genes expressing 70
kDa heat shock proteins were identified in
Cyprinus carpio. The sequence similarities and the intron-interrupted structure of the coding regions indicate that carp Hsc70-1 and Hsc70-2 belong to the Hsp70 cognate subfamily. The expressions of the two
hsc70 genes were followed by semi-quantitative RT-PCR. Both genes are expressed under unstressed conditions in a characteristic tissue-specific manner. Inducibility of the response to elevated temperature, cold shock, and Cd treatment was investigated in the liver and muscle, in whole-animal experiments. Both genes were insensitive to or only weakly induced by the stressors, with two exceptions: Cd treatment resulted in an 11–13-fold enhanced induction of
hsc70-1 in the liver and cold shock enhanced induction of
hsc70-2 in the muscle by 7.5–10-fold. |
| doi_str_mv | 10.1016/S0006-291X(03)01206-3 |
| format | article |
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kDa heat shock proteins were identified in
Cyprinus carpio. The sequence similarities and the intron-interrupted structure of the coding regions indicate that carp Hsc70-1 and Hsc70-2 belong to the Hsp70 cognate subfamily. The expressions of the two
hsc70 genes were followed by semi-quantitative RT-PCR. Both genes are expressed under unstressed conditions in a characteristic tissue-specific manner. Inducibility of the response to elevated temperature, cold shock, and Cd treatment was investigated in the liver and muscle, in whole-animal experiments. Both genes were insensitive to or only weakly induced by the stressors, with two exceptions: Cd treatment resulted in an 11–13-fold enhanced induction of
hsc70-1 in the liver and cold shock enhanced induction of
hsc70-2 in the muscle by 7.5–10-fold.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/S0006-291X(03)01206-3</identifier><identifier>PMID: 12893250</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Animals ; Cadmium - pharmacology ; Carp ; Carps - metabolism ; Cd treatment ; Cold shock ; Cyprinus carpio ; Differential expression ; Gene Expression Regulation ; Hsc70 ; hsc70 gene ; HSC70 Heat-Shock Proteins ; HSP70 Heat-Shock Proteins - biosynthesis ; HSP70 Heat-Shock Proteins - classification ; HSP70 Heat-Shock Proteins - genetics ; Liver - metabolism ; Molecular Sequence Data ; Muscles - metabolism ; Organ Specificity ; Phylogeny ; Protein Isoforms - biosynthesis ; Protein Isoforms - genetics ; Sequence Alignment ; Teleostei ; Temperature ; Tissue Distribution</subject><ispartof>Biochemical and biophysical research communications, 2003-08, Vol.307 (3), p.503-509, Article 503</ispartof><rights>2003 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c444t-33297b0fc2f1800b7c5f5da4b9ff54e6fe624a4bccbf4004ff1836ef0386f263</citedby><cites>FETCH-LOGICAL-c444t-33297b0fc2f1800b7c5f5da4b9ff54e6fe624a4bccbf4004ff1836ef0386f263</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12893250$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ali, Khaled Said</creatorcontrib><creatorcontrib>Dorgai, László</creatorcontrib><creatorcontrib>Ábrahám, Magdolna</creatorcontrib><creatorcontrib>Hermesz, Edit</creatorcontrib><title>Tissue- and stressor-specific differential expression of two hsc70 genes in carp</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Two genes expressing 70
kDa heat shock proteins were identified in
Cyprinus carpio. The sequence similarities and the intron-interrupted structure of the coding regions indicate that carp Hsc70-1 and Hsc70-2 belong to the Hsp70 cognate subfamily. The expressions of the two
hsc70 genes were followed by semi-quantitative RT-PCR. Both genes are expressed under unstressed conditions in a characteristic tissue-specific manner. Inducibility of the response to elevated temperature, cold shock, and Cd treatment was investigated in the liver and muscle, in whole-animal experiments. Both genes were insensitive to or only weakly induced by the stressors, with two exceptions: Cd treatment resulted in an 11–13-fold enhanced induction of
hsc70-1 in the liver and cold shock enhanced induction of
hsc70-2 in the muscle by 7.5–10-fold.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Cadmium - pharmacology</subject><subject>Carp</subject><subject>Carps - metabolism</subject><subject>Cd treatment</subject><subject>Cold shock</subject><subject>Cyprinus carpio</subject><subject>Differential expression</subject><subject>Gene Expression Regulation</subject><subject>Hsc70</subject><subject>hsc70 gene</subject><subject>HSC70 Heat-Shock Proteins</subject><subject>HSP70 Heat-Shock Proteins - biosynthesis</subject><subject>HSP70 Heat-Shock Proteins - classification</subject><subject>HSP70 Heat-Shock Proteins - genetics</subject><subject>Liver - metabolism</subject><subject>Molecular Sequence Data</subject><subject>Muscles - metabolism</subject><subject>Organ Specificity</subject><subject>Phylogeny</subject><subject>Protein Isoforms - biosynthesis</subject><subject>Protein Isoforms - genetics</subject><subject>Sequence Alignment</subject><subject>Teleostei</subject><subject>Temperature</subject><subject>Tissue Distribution</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNqFkE1P3DAQhi3Uqizb_oQinyo4hI4_kmzEoaoQXxJSK3UPvVmOMy5G2SR4vLD8e7LsAqIXTqPRPPPO6GHsq4AjAaL4_gcAikxW4u8BqEMQcuzUDpsIqCCTAvQHNnlBdtke0Q2AELqoPrFdIWeVkjlM2O95IFpixm3XcEoRifqY0YAu-OB4E7zHiF0KtuW4Gtbz0He89zzd9_yaXAn8H3ZIPHTc2Th8Zh-9bQm_bOuUzc9O5ycX2dWv88uTn1eZ01qnTClZlTV4J72YAdSly33eWF1X3ucaC4-F1GPrXO01gPYjpgr0oGaFl4Wasm-b2CH2t0ukZBaBHLat7bBfkilVrmSp1bugmFUaxmdGMN-ALvZEEb0ZYljY-GAEmLVy86TcrH0aUOZJuVkf2N8eWNYLbF63to5H4Pi_YBeSTaPGFG1oX-LpOX71Nv7HZhtHmXcBoyEXsHPYhIgumaYP7zz4CLu2o7c</recordid><startdate>20030801</startdate><enddate>20030801</enddate><creator>Ali, Khaled Said</creator><creator>Dorgai, László</creator><creator>Ábrahám, Magdolna</creator><creator>Hermesz, Edit</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20030801</creationdate><title>Tissue- and stressor-specific differential expression of two hsc70 genes in carp</title><author>Ali, Khaled Said ; 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kDa heat shock proteins were identified in
Cyprinus carpio. The sequence similarities and the intron-interrupted structure of the coding regions indicate that carp Hsc70-1 and Hsc70-2 belong to the Hsp70 cognate subfamily. The expressions of the two
hsc70 genes were followed by semi-quantitative RT-PCR. Both genes are expressed under unstressed conditions in a characteristic tissue-specific manner. Inducibility of the response to elevated temperature, cold shock, and Cd treatment was investigated in the liver and muscle, in whole-animal experiments. Both genes were insensitive to or only weakly induced by the stressors, with two exceptions: Cd treatment resulted in an 11–13-fold enhanced induction of
hsc70-1 in the liver and cold shock enhanced induction of
hsc70-2 in the muscle by 7.5–10-fold.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>12893250</pmid><doi>10.1016/S0006-291X(03)01206-3</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 2003-08, Vol.307 (3), p.503-509, Article 503 |
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| language | eng |
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| source | ScienceDirect Freedom Collection |
| subjects | Amino Acid Sequence Animals Cadmium - pharmacology Carp Carps - metabolism Cd treatment Cold shock Cyprinus carpio Differential expression Gene Expression Regulation Hsc70 hsc70 gene HSC70 Heat-Shock Proteins HSP70 Heat-Shock Proteins - biosynthesis HSP70 Heat-Shock Proteins - classification HSP70 Heat-Shock Proteins - genetics Liver - metabolism Molecular Sequence Data Muscles - metabolism Organ Specificity Phylogeny Protein Isoforms - biosynthesis Protein Isoforms - genetics Sequence Alignment Teleostei Temperature Tissue Distribution |
| title | Tissue- and stressor-specific differential expression of two hsc70 genes in carp |
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