Cloning and characterisation of a putative ST2L homologue from Atlantic salmon ( Salmo salar)

The ST2L receptor is a member of the interleukin-1 (IL-1) receptor family and has previously been cloned from human, mouse, rat and chicken. This orphan receptor has no known physiological role but has been implicated in T helper cell type 2 effector function. We describe in this report the cloning...

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Bibliographic Details
Published in:Fish & shellfish immunology 2003-09, Vol.15 (3), p.211-224
Main Authors: Stansberg, Christine, Subramaniam, Sumathi, Olsen, Litta, Secombes, Christopher J, Cunningham, Charles
Format: Article
Language:English
Subjects:
Animals
Base Sequence
DNA Primers
DNA Probes
Gene Expression
Gene Library
Ig domain
IL-1R family
interleukin 1 receptors
Molecular Sequence Data
Phylogeny
Receptors, Interleukin-1 - genetics
Reverse Transcriptase Polymerase Chain Reaction
Salmo salar
Salmo salar - genetics
Salmo salar - immunology
ST2L
ST2L protein
TIR domain
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Summary:The ST2L receptor is a member of the interleukin-1 (IL-1) receptor family and has previously been cloned from human, mouse, rat and chicken. This orphan receptor has no known physiological role but has been implicated in T helper cell type 2 effector function. We describe in this report the cloning and characterisation of a cDNA encoding a homologue of ST2L in Atlantic salmon ( Salmo salar). The salmon ST2L cDNA is 2364 bp in length and has an open reading frame encoding a polypeptide of 582 amino acids. Similar to other members of the IL-1 receptor (IL-1R) family, the predicted protein has a potential signal peptide, extracellular immunoglobulin-like domains, a short transmembrane region and a characteristic cytoplasmic Toll-IL-1R domain. The predicted protein shows 33% identity and 44% similarity to the chicken ST2L homologue. Phylogenetic analyses cluster the putative salmon ST2L with the chicken and the mammalian ST2L homologues, away from the other members of the IL-1R family. Salmon ST2L is constitutively expressed in brain, white and red blood cells, head kidney, liver, gills and muscle, with highest level of expression in spleen. In vivo stimulation of salmon with lipopolysaccaride does not appear to have a significant effect on expression of the ST2L homologue.
ISSN:1050-4648
1095-9947
DOI:10.1016/S1050-4648(02)00162-6