Association of increased autophagic inclusions labeled for β-galactosidase with fibroblastic aging

Replicative senescence appears after a finite number of cell divisions. After proliferation has ceased, senescent cells remain viable for long periods and metabolic modifications are observed such as lipofuscin accumulation. In order to understand this phenomenon, we examined the emergence of subcel...

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Bibliographic Details
Published in:Experimental gerontology 2003-08, Vol.38 (8), p.887-895
Main Authors: Gerland, Luc-Marie, Peyrol, Simone, Lallemand, Christophe, Branche, Robert, Magaud, Jean-Pierre, Ffrench, Martine
Format: Article
Language:English
Subjects:
Autophagocytosis
Autophagy - physiology
beta-Galactosidase - analysis
Biomarkers - analysis
Cadaverine - analogs & derivatives
Cadaverine - analysis
Cell aging
Cells, Cultured
Cellular Senescence - physiology
Degradative autophagic vacuoles
Fibroblasts
Fibroblasts - chemistry
Fibroblasts - physiology
Humans
Inclusion Bodies - ultrastructure
Lipofuscin - analysis
Microscopy, Electron
Microscopy, Fluorescence
Senescence-associated-β-galactosidase
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Summary:Replicative senescence appears after a finite number of cell divisions. After proliferation has ceased, senescent cells remain viable for long periods and metabolic modifications are observed such as lipofuscin accumulation. In order to understand this phenomenon, we examined the emergence of subcellular modifications corresponding to autophagy in MRC5 normal human fibroblasts. An increase of monodansylcadaverine fluorescence, a specific marker of autophagy, in aging compared to young fibroblasts was observed ( p
ISSN:0531-5565
1873-6815
DOI:10.1016/S0531-5565(03)00132-3