Xenopus Germes encodes a novel germ plasm-associated transcript

A cDNA tag specific for the vegetal pole of early Xenopus embryo was used to isolate a novel cDNA using RACE technique. The corresponding mRNA demonstrated a localization pattern typical for the germ plasm-associated messages, and was, therefore, named Germes. The open reading frame of Germes encode...

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Bibliographic Details
Published in:Gene Expression Patterns 2003-08, Vol.3 (4), p.521-524
Main Authors: Berekelya, Lyubov A., Ponomarev, Maxim B., Luchinskaya, Natalia N., Belyavsky, Alexander V.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
Cytoplasm - metabolism
DNA, Complementary
DNA-Binding Proteins - genetics
Female
Gene Expression Profiling
Gene Expression Regulation, Developmental
Germ Cells - metabolism
Germ plasm
In Situ Hybridization
Localized RNA
Molecular Sequence Data
Oligonucleotide Array Sequence Analysis
Primordial germ cell
RNA Probes
RNA, Messenger - metabolism
Transcription Factors - genetics
Xenopus
Xenopus laevis - embryology
Xenopus laevis - growth & development
Xenopus laevis - metabolism
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Summary:A cDNA tag specific for the vegetal pole of early Xenopus embryo was used to isolate a novel cDNA using RACE technique. The corresponding mRNA demonstrated a localization pattern typical for the germ plasm-associated messages, and was, therefore, named Germes. The open reading frame of Germes encodes a predicted 68 kDa protein with two leucine zipper motifs and a putative EF-hand domain, but otherwise no substantial homology to known proteins. In situ hybridization analysis shows that Germes transcript localizes to the vegetal cortex via the mitochondrial cloud early in oogenesis and segregates with the germ plasm during early embryogenesis. Our data indicate that Germes is a novel germ plasm-specific RNA.
ISSN:1567-133X
1872-7298
DOI:10.1016/S1567-133X(03)00055-3