Loading…
The immunostimulatory activity and stability of grass carp ( Ctenopharyngodon idellus) roe lectin
A hexameric rhamnose-specific lectin with a molecular mass of 205 kDa and exhibiting some N-terminal sequence similarity to other fish lectins was isolated from roe of the grass carp ( Ctenopharyngodon idellus) by affinity chromatography on rhamnose-Sepharose and ion exchange chromatography by fast...
Saved in:
| Published in: | Veterinary Immunology and Immunopathology 2003-08, Vol.94 (3), p.105-112 |
|---|---|
| Main Authors: | , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c458t-24fa549bbdd1f3729d00ed84617a6596fe5a4a089b8aea48d86d210793deca43 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c458t-24fa549bbdd1f3729d00ed84617a6596fe5a4a089b8aea48d86d210793deca43 |
| container_end_page | 112 |
| container_issue | 3 |
| container_start_page | 105 |
| container_title | Veterinary Immunology and Immunopathology |
| container_volume | 94 |
| creator | Ng, T.B. Lam, Y.W. Woo, N.Y.S. |
| description | A hexameric rhamnose-specific lectin with a molecular mass of 205
kDa and exhibiting some N-terminal sequence similarity to other fish lectins was isolated from roe of the grass carp (
Ctenopharyngodon idellus) by affinity chromatography on rhamnose-Sepharose and ion exchange chromatography by fast protein liquid chromatography on a Mono S column. The lectin exhibited mitogenic activity toward murine splenocytes with a potency lower than that of the plant lectin ConA. It exerted a stimulatory effect at a concentration of 10
μg/ml on the phagocytic activity of seabream (
Sparus sarba) macrophages. It was unstable toward heat (temperature ≥40
°C), acid (0.1
M HCl), alkali (0.1
M NaOH), trypsin and succinylation. |
| doi_str_mv | 10.1016/S0165-2427(03)00067-9 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73559030</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0165242703000679</els_id><sourcerecordid>17298032</sourcerecordid><originalsourceid>FETCH-LOGICAL-c458t-24fa549bbdd1f3729d00ed84617a6596fe5a4a089b8aea48d86d210793deca43</originalsourceid><addsrcrecordid>eNqFkU9v3CAQxVHVKrtJ8xFacaqSg9vBgA2nKFrlnxSph-4dYTPOUtnGBTtSvn3Y7Ko55gKM9Jt5w3uEfGPwkwGrfv3JhyxKUdYXwC8BoKoL_Ymsmap5UUouPpP1f2RFTlP6myGplTohK1Zq0ALqNbHbHVI_DMsY0uyHpbdziC_UtrN_9nN-jI6m2Ta-31eho0_RpkRbGyd6QTczjmHa2fgyPgUXRuod9v2SLmkMSHvMU8av5Etn-4Tnx_uMbG9vtpv74vH33cPm-rFohVRzXrOzUuimcY51vC61A0CnRMVqW0lddSitsKB0oyxaoZyqXMmg1txhawU_Iz8OY6cY_i2YZjP41OZt7IhhSabmUmrg8CHIsrYCXmZQHsA2hpQidmaKfsh_NQzMPgPzloHZG2yAm7cMjM59348CSzOge-86mp6BqwOA2Y5nj9Gk1uPYovMxW2Zc8B9IvAKWmZd9</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17298032</pqid></control><display><type>article</type><title>The immunostimulatory activity and stability of grass carp ( Ctenopharyngodon idellus) roe lectin</title><source>PubMed (Medline)</source><source>ScienceDirect Freedom Collection 2022-2024</source><source>Wiley-Blackwell Read & Publish Collection</source><creator>Ng, T.B. ; Lam, Y.W. ; Woo, N.Y.S.</creator><creatorcontrib>Ng, T.B. ; Lam, Y.W. ; Woo, N.Y.S.</creatorcontrib><description>A hexameric rhamnose-specific lectin with a molecular mass of 205
kDa and exhibiting some N-terminal sequence similarity to other fish lectins was isolated from roe of the grass carp (
Ctenopharyngodon idellus) by affinity chromatography on rhamnose-Sepharose and ion exchange chromatography by fast protein liquid chromatography on a Mono S column. The lectin exhibited mitogenic activity toward murine splenocytes with a potency lower than that of the plant lectin ConA. It exerted a stimulatory effect at a concentration of 10
μg/ml on the phagocytic activity of seabream (
Sparus sarba) macrophages. It was unstable toward heat (temperature ≥40
°C), acid (0.1
M HCl), alkali (0.1
M NaOH), trypsin and succinylation.</description><identifier>ISSN: 0165-2427</identifier><identifier>EISSN: 1873-2534</identifier><identifier>EISSN: 1365-2567</identifier><identifier>DOI: 10.1016/S0165-2427(03)00067-9</identifier><identifier>PMID: 12909407</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Adjuvants, Immunologic - isolation & purification ; Adjuvants, Immunologic - metabolism ; Adjuvants, Immunologic - pharmacology ; Amino Acid Sequence ; Animals ; Carps - immunology ; Carps - metabolism ; Chromatography, Affinity - veterinary ; Chromatography, Ion Exchange - veterinary ; Ctenopharyngodon idella ; Ctenopharyngodon idellus ; Drug Stability ; Freshwater ; Grass carp ; Hemagglutination - immunology ; Hot Temperature ; Hydrogen-Ion Concentration ; Lectin ; Lectins - immunology ; Lectins - isolation & purification ; Lectins - metabolism ; Lectins - pharmacology ; Mice ; Mice, Inbred ICR ; Molecular Sequence Data ; Rhamnose - metabolism ; Rhamnose-binding ; Sea Bream ; Sequence Alignment ; Sequence Analysis, Protein ; Sparus sarba ; Spleen - cytology ; Spleen - drug effects ; Trypsin - metabolism</subject><ispartof>Veterinary Immunology and Immunopathology, 2003-08, Vol.94 (3), p.105-112</ispartof><rights>2003 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c458t-24fa549bbdd1f3729d00ed84617a6596fe5a4a089b8aea48d86d210793deca43</citedby><cites>FETCH-LOGICAL-c458t-24fa549bbdd1f3729d00ed84617a6596fe5a4a089b8aea48d86d210793deca43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12909407$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ng, T.B.</creatorcontrib><creatorcontrib>Lam, Y.W.</creatorcontrib><creatorcontrib>Woo, N.Y.S.</creatorcontrib><title>The immunostimulatory activity and stability of grass carp ( Ctenopharyngodon idellus) roe lectin</title><title>Veterinary Immunology and Immunopathology</title><addtitle>Vet Immunol Immunopathol</addtitle><description>A hexameric rhamnose-specific lectin with a molecular mass of 205
kDa and exhibiting some N-terminal sequence similarity to other fish lectins was isolated from roe of the grass carp (
Ctenopharyngodon idellus) by affinity chromatography on rhamnose-Sepharose and ion exchange chromatography by fast protein liquid chromatography on a Mono S column. The lectin exhibited mitogenic activity toward murine splenocytes with a potency lower than that of the plant lectin ConA. It exerted a stimulatory effect at a concentration of 10
μg/ml on the phagocytic activity of seabream (
Sparus sarba) macrophages. It was unstable toward heat (temperature ≥40
°C), acid (0.1
M HCl), alkali (0.1
M NaOH), trypsin and succinylation.</description><subject>Adjuvants, Immunologic - isolation & purification</subject><subject>Adjuvants, Immunologic - metabolism</subject><subject>Adjuvants, Immunologic - pharmacology</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Carps - immunology</subject><subject>Carps - metabolism</subject><subject>Chromatography, Affinity - veterinary</subject><subject>Chromatography, Ion Exchange - veterinary</subject><subject>Ctenopharyngodon idella</subject><subject>Ctenopharyngodon idellus</subject><subject>Drug Stability</subject><subject>Freshwater</subject><subject>Grass carp</subject><subject>Hemagglutination - immunology</subject><subject>Hot Temperature</subject><subject>Hydrogen-Ion Concentration</subject><subject>Lectin</subject><subject>Lectins - immunology</subject><subject>Lectins - isolation & purification</subject><subject>Lectins - metabolism</subject><subject>Lectins - pharmacology</subject><subject>Mice</subject><subject>Mice, Inbred ICR</subject><subject>Molecular Sequence Data</subject><subject>Rhamnose - metabolism</subject><subject>Rhamnose-binding</subject><subject>Sea Bream</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, Protein</subject><subject>Sparus sarba</subject><subject>Spleen - cytology</subject><subject>Spleen - drug effects</subject><subject>Trypsin - metabolism</subject><issn>0165-2427</issn><issn>1873-2534</issn><issn>1365-2567</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNqFkU9v3CAQxVHVKrtJ8xFacaqSg9vBgA2nKFrlnxSph-4dYTPOUtnGBTtSvn3Y7Ko55gKM9Jt5w3uEfGPwkwGrfv3JhyxKUdYXwC8BoKoL_Ymsmap5UUouPpP1f2RFTlP6myGplTohK1Zq0ALqNbHbHVI_DMsY0uyHpbdziC_UtrN_9nN-jI6m2Ta-31eho0_RpkRbGyd6QTczjmHa2fgyPgUXRuod9v2SLmkMSHvMU8av5Etn-4Tnx_uMbG9vtpv74vH33cPm-rFohVRzXrOzUuimcY51vC61A0CnRMVqW0lddSitsKB0oyxaoZyqXMmg1txhawU_Iz8OY6cY_i2YZjP41OZt7IhhSabmUmrg8CHIsrYCXmZQHsA2hpQidmaKfsh_NQzMPgPzloHZG2yAm7cMjM59348CSzOge-86mp6BqwOA2Y5nj9Gk1uPYovMxW2Zc8B9IvAKWmZd9</recordid><startdate>20030815</startdate><enddate>20030815</enddate><creator>Ng, T.B.</creator><creator>Lam, Y.W.</creator><creator>Woo, N.Y.S.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20030815</creationdate><title>The immunostimulatory activity and stability of grass carp ( Ctenopharyngodon idellus) roe lectin</title><author>Ng, T.B. ; Lam, Y.W. ; Woo, N.Y.S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c458t-24fa549bbdd1f3729d00ed84617a6596fe5a4a089b8aea48d86d210793deca43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Adjuvants, Immunologic - isolation & purification</topic><topic>Adjuvants, Immunologic - metabolism</topic><topic>Adjuvants, Immunologic - pharmacology</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Carps - immunology</topic><topic>Carps - metabolism</topic><topic>Chromatography, Affinity - veterinary</topic><topic>Chromatography, Ion Exchange - veterinary</topic><topic>Ctenopharyngodon idella</topic><topic>Ctenopharyngodon idellus</topic><topic>Drug Stability</topic><topic>Freshwater</topic><topic>Grass carp</topic><topic>Hemagglutination - immunology</topic><topic>Hot Temperature</topic><topic>Hydrogen-Ion Concentration</topic><topic>Lectin</topic><topic>Lectins - immunology</topic><topic>Lectins - isolation & purification</topic><topic>Lectins - metabolism</topic><topic>Lectins - pharmacology</topic><topic>Mice</topic><topic>Mice, Inbred ICR</topic><topic>Molecular Sequence Data</topic><topic>Rhamnose - metabolism</topic><topic>Rhamnose-binding</topic><topic>Sea Bream</topic><topic>Sequence Alignment</topic><topic>Sequence Analysis, Protein</topic><topic>Sparus sarba</topic><topic>Spleen - cytology</topic><topic>Spleen - drug effects</topic><topic>Trypsin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ng, T.B.</creatorcontrib><creatorcontrib>Lam, Y.W.</creatorcontrib><creatorcontrib>Woo, N.Y.S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary Immunology and Immunopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ng, T.B.</au><au>Lam, Y.W.</au><au>Woo, N.Y.S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The immunostimulatory activity and stability of grass carp ( Ctenopharyngodon idellus) roe lectin</atitle><jtitle>Veterinary Immunology and Immunopathology</jtitle><addtitle>Vet Immunol Immunopathol</addtitle><date>2003-08-15</date><risdate>2003</risdate><volume>94</volume><issue>3</issue><spage>105</spage><epage>112</epage><pages>105-112</pages><issn>0165-2427</issn><eissn>1873-2534</eissn><eissn>1365-2567</eissn><abstract>A hexameric rhamnose-specific lectin with a molecular mass of 205
kDa and exhibiting some N-terminal sequence similarity to other fish lectins was isolated from roe of the grass carp (
Ctenopharyngodon idellus) by affinity chromatography on rhamnose-Sepharose and ion exchange chromatography by fast protein liquid chromatography on a Mono S column. The lectin exhibited mitogenic activity toward murine splenocytes with a potency lower than that of the plant lectin ConA. It exerted a stimulatory effect at a concentration of 10
μg/ml on the phagocytic activity of seabream (
Sparus sarba) macrophages. It was unstable toward heat (temperature ≥40
°C), acid (0.1
M HCl), alkali (0.1
M NaOH), trypsin and succinylation.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>12909407</pmid><doi>10.1016/S0165-2427(03)00067-9</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0165-2427 |
| ispartof | Veterinary Immunology and Immunopathology, 2003-08, Vol.94 (3), p.105-112 |
| issn | 0165-2427 1873-2534 1365-2567 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73559030 |
| source | PubMed (Medline); ScienceDirect Freedom Collection 2022-2024; Wiley-Blackwell Read & Publish Collection |
| subjects | Adjuvants, Immunologic - isolation & purification Adjuvants, Immunologic - metabolism Adjuvants, Immunologic - pharmacology Amino Acid Sequence Animals Carps - immunology Carps - metabolism Chromatography, Affinity - veterinary Chromatography, Ion Exchange - veterinary Ctenopharyngodon idella Ctenopharyngodon idellus Drug Stability Freshwater Grass carp Hemagglutination - immunology Hot Temperature Hydrogen-Ion Concentration Lectin Lectins - immunology Lectins - isolation & purification Lectins - metabolism Lectins - pharmacology Mice Mice, Inbred ICR Molecular Sequence Data Rhamnose - metabolism Rhamnose-binding Sea Bream Sequence Alignment Sequence Analysis, Protein Sparus sarba Spleen - cytology Spleen - drug effects Trypsin - metabolism |
| title | The immunostimulatory activity and stability of grass carp ( Ctenopharyngodon idellus) roe lectin |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T10%3A37%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20immunostimulatory%20activity%20and%20stability%20of%20grass%20carp%20(%20Ctenopharyngodon%20idellus)%20roe%20lectin&rft.jtitle=Veterinary%20Immunology%20and%20Immunopathology&rft.au=Ng,%20T.B.&rft.date=2003-08-15&rft.volume=94&rft.issue=3&rft.spage=105&rft.epage=112&rft.pages=105-112&rft.issn=0165-2427&rft.eissn=1873-2534&rft_id=info:doi/10.1016/S0165-2427(03)00067-9&rft_dat=%3Cproquest_cross%3E17298032%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c458t-24fa549bbdd1f3729d00ed84617a6596fe5a4a089b8aea48d86d210793deca43%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=17298032&rft_id=info:pmid/12909407&rfr_iscdi=true |