Fractionation, structural studies, and immunological characterization of the semi-synthetic Quillaja saponins derivative GPI-0100

Unfractionated GPI-0100 (UFGPI-0100) containing semi-synthetic derivatives of deacylated Quillaja saponins (DS saponins) modified at the glucuronic acid residue was resolved by reverse phase low-pressure liquid chromatography (RP-LPLC) into two fractions, RP18-1 and RP18-2, with different compositio...

Full description

Saved in:
Bibliographic Details
Published in:Vaccine 2003-09, Vol.21 (25-26), p.3961-3971
Main Authors: Marciani, Dante J, Reynolds, Robert C, Pathak, Ashish K, Finley-Woodman, Kyra, May, Richard D
Format: Article
Language:English
Subjects:
Adducts
Adjuvant
Adjuvanticity
Adjuvants, Immunologic - chemistry
Adjuvants, Immunologic - pharmacology
Alum Compounds
Animals
Antigens
Antigens - immunology
Arthritis
Carbohydrate Sequence
Cell Line
Chromatography
Chromatography, Liquid
Cytotoxicity
Derivatives
E coli
Enzyme-Linked Immunosorbent Assay
Fatty acids
Female
Fractionation
Fractions
GPI-0100
Immune system
Immunity
Immunity, Cellular
Immunoglobulin G
Immunoglobulin G - biosynthesis
Immunology
Indicators and Reagents
Interferon-gamma - biosynthesis
Interleukin 2
Interleukin-2 - biosynthesis
Liquid chromatography
Low pressure
Lymphocytes T
Mass Spectrometry
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Micelles
Molecular Sequence Data
Ovalbumin - immunology
Quillaja
Quillaja - chemistry
Quillaja saponins
Saponin analogs
Saponins
Saponins - chemistry
Saponins - immunology
Saponins - pharmacology
Structural analysis
Th1 Cells - immunology
Toxicity
Vaccines
γ-Interferon
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Unfractionated GPI-0100 (UFGPI-0100) containing semi-synthetic derivatives of deacylated Quillaja saponins (DS saponins) modified at the glucuronic acid residue was resolved by reverse phase low-pressure liquid chromatography (RP-LPLC) into two fractions, RP18-1 and RP18-2, with different compositions and adjuvanticity. The fraction RP18-1 contained DS saponin adducts of N-dicyclohexylurea, and stimulated Th2 immunity with production of IgG1, while the RP18-2 fraction contained the dodecylamide derivatives of DS saponins and stimulated Th1 immunity with production of IgG2a, IFN-γ, IL-2, and CTL. The strong immune stimulatory properties of RP18-2, relative to RP18-1, and the formation of RP18-1/RP18-2 mixed micelles may account for the effective stimulation of Th1 immunity by UFGPI-0100. UFGPI-0100 was free of acylated quillaja saponin components, including the more stable QS-7.
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(03)00298-6