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Fractionation, structural studies, and immunological characterization of the semi-synthetic Quillaja saponins derivative GPI-0100
Unfractionated GPI-0100 (UFGPI-0100) containing semi-synthetic derivatives of deacylated Quillaja saponins (DS saponins) modified at the glucuronic acid residue was resolved by reverse phase low-pressure liquid chromatography (RP-LPLC) into two fractions, RP18-1 and RP18-2, with different compositio...
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| Published in: | Vaccine 2003-09, Vol.21 (25-26), p.3961-3971 |
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| container_end_page | 3971 |
| container_issue | 25-26 |
| container_start_page | 3961 |
| container_title | Vaccine |
| container_volume | 21 |
| creator | Marciani, Dante J Reynolds, Robert C Pathak, Ashish K Finley-Woodman, Kyra May, Richard D |
| description | Unfractionated GPI-0100 (UFGPI-0100) containing semi-synthetic derivatives of deacylated Quillaja saponins (DS saponins) modified at the glucuronic acid residue was resolved by reverse phase low-pressure liquid chromatography (RP-LPLC) into two fractions, RP18-1 and RP18-2, with different compositions and adjuvanticity. The fraction RP18-1 contained DS saponin adducts of N-dicyclohexylurea, and stimulated Th2 immunity with production of IgG1, while the RP18-2 fraction contained the dodecylamide derivatives of DS saponins and stimulated Th1 immunity with production of IgG2a, IFN-γ, IL-2, and CTL. The strong immune stimulatory properties of RP18-2, relative to RP18-1, and the formation of RP18-1/RP18-2 mixed micelles may account for the effective stimulation of Th1 immunity by UFGPI-0100. UFGPI-0100 was free of acylated quillaja saponin components, including the more stable QS-7. |
| doi_str_mv | 10.1016/S0264-410X(03)00298-6 |
| format | article |
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The fraction RP18-1 contained DS saponin adducts of N-dicyclohexylurea, and stimulated Th2 immunity with production of IgG1, while the RP18-2 fraction contained the dodecylamide derivatives of DS saponins and stimulated Th1 immunity with production of IgG2a, IFN-γ, IL-2, and CTL. The strong immune stimulatory properties of RP18-2, relative to RP18-1, and the formation of RP18-1/RP18-2 mixed micelles may account for the effective stimulation of Th1 immunity by UFGPI-0100. UFGPI-0100 was free of acylated quillaja saponin components, including the more stable QS-7.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/S0264-410X(03)00298-6</identifier><identifier>PMID: 12922132</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Adducts ; Adjuvant ; Adjuvanticity ; Adjuvants, Immunologic - chemistry ; Adjuvants, Immunologic - pharmacology ; Alum Compounds ; Animals ; Antigens ; Antigens - immunology ; Arthritis ; Carbohydrate Sequence ; Cell Line ; Chromatography ; Chromatography, Liquid ; Cytotoxicity ; Derivatives ; E coli ; Enzyme-Linked Immunosorbent Assay ; Fatty acids ; Female ; Fractionation ; Fractions ; GPI-0100 ; Immune system ; Immunity ; Immunity, Cellular ; Immunoglobulin G ; Immunoglobulin G - biosynthesis ; Immunology ; Indicators and Reagents ; Interferon-gamma - biosynthesis ; Interleukin 2 ; Interleukin-2 - biosynthesis ; Liquid chromatography ; Low pressure ; Lymphocytes T ; Mass Spectrometry ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Micelles ; Molecular Sequence Data ; Ovalbumin - immunology ; Quillaja ; Quillaja - chemistry ; Quillaja saponins ; Saponin analogs ; Saponins ; Saponins - chemistry ; Saponins - immunology ; Saponins - pharmacology ; Structural analysis ; Th1 Cells - immunology ; Toxicity ; Vaccines ; γ-Interferon</subject><ispartof>Vaccine, 2003-09, Vol.21 (25-26), p.3961-3971</ispartof><rights>2003 Elsevier Science Ltd</rights><rights>Copyright Elsevier Limited Sep 8, 2003</rights><rights>2003. Elsevier Science Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c535t-590bff44de8fe3637758295e58f83d64af3cd8708f9453d4555981b04e44d6853</citedby><cites>FETCH-LOGICAL-c535t-590bff44de8fe3637758295e58f83d64af3cd8708f9453d4555981b04e44d6853</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12922132$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Marciani, Dante J</creatorcontrib><creatorcontrib>Reynolds, Robert C</creatorcontrib><creatorcontrib>Pathak, Ashish K</creatorcontrib><creatorcontrib>Finley-Woodman, Kyra</creatorcontrib><creatorcontrib>May, Richard D</creatorcontrib><title>Fractionation, structural studies, and immunological characterization of the semi-synthetic Quillaja saponins derivative GPI-0100</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Unfractionated GPI-0100 (UFGPI-0100) containing semi-synthetic derivatives of deacylated Quillaja saponins (DS saponins) modified at the glucuronic acid residue was resolved by reverse phase low-pressure liquid chromatography (RP-LPLC) into two fractions, RP18-1 and RP18-2, with different compositions and adjuvanticity. The fraction RP18-1 contained DS saponin adducts of N-dicyclohexylurea, and stimulated Th2 immunity with production of IgG1, while the RP18-2 fraction contained the dodecylamide derivatives of DS saponins and stimulated Th1 immunity with production of IgG2a, IFN-γ, IL-2, and CTL. The strong immune stimulatory properties of RP18-2, relative to RP18-1, and the formation of RP18-1/RP18-2 mixed micelles may account for the effective stimulation of Th1 immunity by UFGPI-0100. UFGPI-0100 was free of acylated quillaja saponin components, including the more stable QS-7.</description><subject>Adducts</subject><subject>Adjuvant</subject><subject>Adjuvanticity</subject><subject>Adjuvants, Immunologic - chemistry</subject><subject>Adjuvants, Immunologic - pharmacology</subject><subject>Alum Compounds</subject><subject>Animals</subject><subject>Antigens</subject><subject>Antigens - immunology</subject><subject>Arthritis</subject><subject>Carbohydrate Sequence</subject><subject>Cell Line</subject><subject>Chromatography</subject><subject>Chromatography, Liquid</subject><subject>Cytotoxicity</subject><subject>Derivatives</subject><subject>E coli</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fatty acids</subject><subject>Female</subject><subject>Fractionation</subject><subject>Fractions</subject><subject>GPI-0100</subject><subject>Immune system</subject><subject>Immunity</subject><subject>Immunity, Cellular</subject><subject>Immunoglobulin G</subject><subject>Immunoglobulin G - biosynthesis</subject><subject>Immunology</subject><subject>Indicators and Reagents</subject><subject>Interferon-gamma - biosynthesis</subject><subject>Interleukin 2</subject><subject>Interleukin-2 - biosynthesis</subject><subject>Liquid chromatography</subject><subject>Low pressure</subject><subject>Lymphocytes T</subject><subject>Mass Spectrometry</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred C57BL</subject><subject>Micelles</subject><subject>Molecular Sequence Data</subject><subject>Ovalbumin - immunology</subject><subject>Quillaja</subject><subject>Quillaja - chemistry</subject><subject>Quillaja saponins</subject><subject>Saponin analogs</subject><subject>Saponins</subject><subject>Saponins - chemistry</subject><subject>Saponins - immunology</subject><subject>Saponins - pharmacology</subject><subject>Structural analysis</subject><subject>Th1 Cells - immunology</subject><subject>Toxicity</subject><subject>Vaccines</subject><subject>γ-Interferon</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNqNkl1rFTEQhhdR7LH6E5SAIBW6OrPJZJMrkWJroaCignchJ5u1OezHabJ7oN75z835QMGL6k0ykOeZJMxbFE8RXiGgfP0ZKilKgfDtBPhLgEqrUt4rFqhqXlaE6n6x-I0cFY9SWgEAcdQPiyOsdFUhrxbFz_No3RTGwW6XU5amOLtpjrbL5dwEn06ZHRoW-n4exm78Hlw-ctd2q_kYfuw8NrZsuvYs-T6U6XbI9RQc-zSHrrMry5Jdj0MYEmuyssnKxrOLj5clIMDj4kFru-SfHPbj4uv5uy9n78urDxeXZ2-vSkecppI0LNtWiMar1nPJ65pUpcmTahVvpLAtd42qQbVaEG8EEWmFSxA-O1IRPy5e7Puu43gz-zSZPiTn8wMHP87J1JykJMkzeHIniCQBuay1_g8UoK5VxTGjz_9CV-Mch_xjU6FWgEgo7qKQhOYcJdWZoj3l4phS9K1Zx9DbeGsQzDYdZpcOsx29AW526TAye88O3edl75s_1iEOGXizB3wexCb4aJILfnC-CdG7yTRj-McVvwA1BchI</recordid><startdate>20030908</startdate><enddate>20030908</enddate><creator>Marciani, Dante J</creator><creator>Reynolds, Robert C</creator><creator>Pathak, Ashish K</creator><creator>Finley-Woodman, Kyra</creator><creator>May, Richard D</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7T2</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88C</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0R</scope><scope>M0S</scope><scope>M0T</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20030908</creationdate><title>Fractionation, structural studies, and immunological characterization of the semi-synthetic Quillaja saponins derivative GPI-0100</title><author>Marciani, Dante J ; Reynolds, Robert C ; Pathak, Ashish K ; Finley-Woodman, Kyra ; May, Richard D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c535t-590bff44de8fe3637758295e58f83d64af3cd8708f9453d4555981b04e44d6853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Adducts</topic><topic>Adjuvant</topic><topic>Adjuvanticity</topic><topic>Adjuvants, Immunologic - chemistry</topic><topic>Adjuvants, Immunologic - pharmacology</topic><topic>Alum Compounds</topic><topic>Animals</topic><topic>Antigens</topic><topic>Antigens - immunology</topic><topic>Arthritis</topic><topic>Carbohydrate Sequence</topic><topic>Cell Line</topic><topic>Chromatography</topic><topic>Chromatography, Liquid</topic><topic>Cytotoxicity</topic><topic>Derivatives</topic><topic>E coli</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fatty acids</topic><topic>Female</topic><topic>Fractionation</topic><topic>Fractions</topic><topic>GPI-0100</topic><topic>Immune system</topic><topic>Immunity</topic><topic>Immunity, Cellular</topic><topic>Immunoglobulin G</topic><topic>Immunoglobulin G - biosynthesis</topic><topic>Immunology</topic><topic>Indicators and Reagents</topic><topic>Interferon-gamma - biosynthesis</topic><topic>Interleukin 2</topic><topic>Interleukin-2 - biosynthesis</topic><topic>Liquid chromatography</topic><topic>Low pressure</topic><topic>Lymphocytes T</topic><topic>Mass Spectrometry</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Inbred C57BL</topic><topic>Micelles</topic><topic>Molecular Sequence Data</topic><topic>Ovalbumin - 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Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Marciani, Dante J</au><au>Reynolds, Robert C</au><au>Pathak, Ashish K</au><au>Finley-Woodman, Kyra</au><au>May, Richard D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fractionation, structural studies, and immunological characterization of the semi-synthetic Quillaja saponins derivative GPI-0100</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2003-09-08</date><risdate>2003</risdate><volume>21</volume><issue>25-26</issue><spage>3961</spage><epage>3971</epage><pages>3961-3971</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><abstract>Unfractionated GPI-0100 (UFGPI-0100) containing semi-synthetic derivatives of deacylated Quillaja saponins (DS saponins) modified at the glucuronic acid residue was resolved by reverse phase low-pressure liquid chromatography (RP-LPLC) into two fractions, RP18-1 and RP18-2, with different compositions and adjuvanticity. The fraction RP18-1 contained DS saponin adducts of N-dicyclohexylurea, and stimulated Th2 immunity with production of IgG1, while the RP18-2 fraction contained the dodecylamide derivatives of DS saponins and stimulated Th1 immunity with production of IgG2a, IFN-γ, IL-2, and CTL. The strong immune stimulatory properties of RP18-2, relative to RP18-1, and the formation of RP18-1/RP18-2 mixed micelles may account for the effective stimulation of Th1 immunity by UFGPI-0100. UFGPI-0100 was free of acylated quillaja saponin components, including the more stable QS-7.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>12922132</pmid><doi>10.1016/S0264-410X(03)00298-6</doi><tpages>11</tpages></addata></record> |
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| subjects | Adducts Adjuvant Adjuvanticity Adjuvants, Immunologic - chemistry Adjuvants, Immunologic - pharmacology Alum Compounds Animals Antigens Antigens - immunology Arthritis Carbohydrate Sequence Cell Line Chromatography Chromatography, Liquid Cytotoxicity Derivatives E coli Enzyme-Linked Immunosorbent Assay Fatty acids Female Fractionation Fractions GPI-0100 Immune system Immunity Immunity, Cellular Immunoglobulin G Immunoglobulin G - biosynthesis Immunology Indicators and Reagents Interferon-gamma - biosynthesis Interleukin 2 Interleukin-2 - biosynthesis Liquid chromatography Low pressure Lymphocytes T Mass Spectrometry Mice Mice, Inbred BALB C Mice, Inbred C57BL Micelles Molecular Sequence Data Ovalbumin - immunology Quillaja Quillaja - chemistry Quillaja saponins Saponin analogs Saponins Saponins - chemistry Saponins - immunology Saponins - pharmacology Structural analysis Th1 Cells - immunology Toxicity Vaccines γ-Interferon |
| title | Fractionation, structural studies, and immunological characterization of the semi-synthetic Quillaja saponins derivative GPI-0100 |
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