Global analysis of G-protein-coupled receptor signaling in human tissues

• Published in: FEBS letters 2003-08, Vol.550 (1), p.11-17
• Main Authors: Hakak, Yaron, Shrestha, Devin, Goegel, Moira C., Behan, Dominic P., Chalmers, Derek T.
• Format: Article
• Language: English
• Subjects: Animals; Cluster Analysis; CNS, central nervous system; G-protein; G-protein, GTP-binding protein; Gene expression; GPCR; GPCR, G-protein-coupled receptor; GTP-Binding Proteins - metabolism; Humans; Mice; Mice, Inbred C57BL; Microarray; Oligonucleotide Array Sequence Analysis; Organ Specificity; RAMP, receptor activity modifying protein; Receptors, Cell Surface - genetics; Receptors, Cell Surface - metabolism; RGS, regulator of G-protein signaling; Signal Transduction - physiology
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/S0014-5793(03)00762-2

The G-protein-coupled receptor (GPCR) family mediates a host of cell–cell communications upon activation by diverse ligands. Numerous GPCRs have been shown to display anatomically selective patterns of gene expression, however, our understanding of the complexity of GPCR signaling within human tissues remains unclear. In an effort to characterize global patterns of GPCR signaling in the human body, microarray analysis was performed on a large panel of tissues to monitor the gene expression levels of the receptors as well as related signaling and regulatory molecules. Analysis of the data revealed complex signaling networks in many tissue types, with tissue-specific patterns of gene expression observed for the majority of the receptors and a number of components and regulators of GPCR signaling.