Estrogen modulates ClC-2 chloride channel gene expression in rat kidney

ClC-2 is a CLC family member of chloride channels sensitive to changes in cell volume, pH and voltage. The ClC-2 is widely distributed along the nephron although in the kidney its role still not well understood. Aldosterone studies suggest that ClC-2 expression in the kidney may be hormonally regula...

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Published in:Pflügers Archiv 2003-08, Vol.446 (5), p.593-599
Main Authors: Nascimento, Danielle S, Reis, Carlos U, Goldenberg, Regina C, Ortiga-Carvalho, Tânia M, Pazos-Moura, Carmen C, Guggino, Sandra E, Guggino, William B, Morales, Marcelo M
Format: Article
Language:English
Subjects:
Animals
Blotting, Western
Cells, Cultured
Chloride Channels - genetics
Estradiol - blood
Estradiol - pharmacology
Estrogens
Female
Gene Expression - drug effects
Kidney Tubules, Proximal - cytology
Kidney Tubules, Proximal - physiology
Kidneys
Nephrons - physiology
Ovariectomy
Rats
Rats, Wistar
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - analysis
Rodents
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Summary:ClC-2 is a CLC family member of chloride channels sensitive to changes in cell volume, pH and voltage. The ClC-2 is widely distributed along the nephron although in the kidney its role still not well understood. Aldosterone studies suggest that ClC-2 expression in the kidney may be hormonally regulated. To explore the possibility that estrogen control ClC-2 expression, we investigated whether its expression changed in the kidney of female Wistar rats subjected to ovariectomy with or without near-physiological or high doses of 17beta-estradiol benzoate treatment for 10 days. Total RNA isolated from rat kidney and dissected nephron segments was analyzed by ribonuclease protection assay and/or a semi-quantitative RT-PCR. The renal ClC-2 protein expression was analyzed by Western blot. The decreased renal expression of ClC-2 mRNA and protein observed in ovariectomized rats was restored to control levels after treatment with low doses of estradiol. Higher dose estradiol lead to an even greater increase in ClC-2 mRNA and protein expression. This change in overall expression was shown to be caused by the modulation of ClC-2 mRNA expression in the proximal tubule. These results suggest that ClC-2 may be involved in estrogen-induced Cl(-) transport in rat kidney.
ISSN:0031-6768
1432-2013
DOI:10.1007/s00424-003-1095-y