Immunohistochemical localization of cholinergic nerve terminals

Most of the published light-microscopic methods for the localization of cholinergic nerve pathways present various difficulties of interpretation. The production and characterization of an antiserum that binds specifically to cholinergic terminals is described. The antiserum was raised to small syna...

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Bibliographic Details
Published in:Cell and tissue research 1981-01, Vol.218 (2), p.355-373
Main Authors: Jones, R T, Walker, J H, Richardson, P J, Fox, G Q, Whittaker, V P
Format: Article
Language:English
Subjects:
acetylchlorine
Acetylcholinesterase - metabolism
Animals
antisera
Complement Fixation Tests
Diaphragm - innervation
Electric Organ - innervation
Fishes
Fluorescent Antibody Technique
Ganglia, Sympathetic - ultrastructure
Guinea Pigs
Hippocampus - ultrastructure
Ileum - innervation
Parasympathetic Nervous System - ultrastructure
Rats
synaptosomes
Synaptosomes - ultrastructure
Torpedo marmorata
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Summary:Most of the published light-microscopic methods for the localization of cholinergic nerve pathways present various difficulties of interpretation. The production and characterization of an antiserum that binds specifically to cholinergic terminals is described. The antiserum was raised to small synaptosomes prepared from the purely cholinergic electric organ of Torpedo marmorata. It was shown to lyse cholinergic synaptosomes in a mixed population derived from guinea-pig cortex. After partial purification by adsorption onto nonspecific antigens, It was used to label nerve endings in several tissues of Torpedo, rat and guinea pigs using indirect immunofluorescence histochemistry. The antiserum appears to provide a highly specific means of localizing cholinergic nerve endings in these tissues.
ISSN:0302-766X
1432-0878
DOI:10.1007/BF00210350