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Immunohistochemical localization of cholinergic nerve terminals

Most of the published light-microscopic methods for the localization of cholinergic nerve pathways present various difficulties of interpretation. The production and characterization of an antiserum that binds specifically to cholinergic terminals is described. The antiserum was raised to small syna...

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Published in:	Cell and tissue research 1981-01, Vol.218 (2), p.355-373
Main Authors:	Jones, R T, Walker, J H, Richardson, P J, Fox, G Q, Whittaker, V P
Format:	Article
Language:	English
Subjects:	acetylchlorine Acetylcholinesterase - metabolism Animals antisera Complement Fixation Tests Diaphragm - innervation Electric Organ - innervation Fishes Fluorescent Antibody Technique Ganglia, Sympathetic - ultrastructure Guinea Pigs Hippocampus - ultrastructure Ileum - innervation Parasympathetic Nervous System - ultrastructure Rats synaptosomes Synaptosomes - ultrastructure Torpedo marmorata
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cited_by	cdi_FETCH-LOGICAL-c313t-a34430dd4f67927e3bdae3d3fe52188edf6b727e31b1ea92cf31d4674384cb7b3
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container_end_page	373
container_issue	2
container_start_page	355
container_title	Cell and tissue research
container_volume	218
creator	Jones, R T Walker, J H Richardson, P J Fox, G Q Whittaker, V P
description	Most of the published light-microscopic methods for the localization of cholinergic nerve pathways present various difficulties of interpretation. The production and characterization of an antiserum that binds specifically to cholinergic terminals is described. The antiserum was raised to small synaptosomes prepared from the purely cholinergic electric organ of Torpedo marmorata. It was shown to lyse cholinergic synaptosomes in a mixed population derived from guinea-pig cortex. After partial purification by adsorption onto nonspecific antigens, It was used to label nerve endings in several tissues of Torpedo, rat and guinea pigs using indirect immunofluorescence histochemistry. The antiserum appears to provide a highly specific means of localizing cholinergic nerve endings in these tissues.
doi_str_mv	10.1007/BF00210350
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The production and characterization of an antiserum that binds specifically to cholinergic terminals is described. The antiserum was raised to small synaptosomes prepared from the purely cholinergic electric organ of Torpedo marmorata. It was shown to lyse cholinergic synaptosomes in a mixed population derived from guinea-pig cortex. After partial purification by adsorption onto nonspecific antigens, It was used to label nerve endings in several tissues of Torpedo, rat and guinea pigs using indirect immunofluorescence histochemistry. The antiserum appears to provide a highly specific means of localizing cholinergic nerve endings in these tissues.</description><identifier>ISSN: 0302-766X</identifier><identifier>EISSN: 1432-0878</identifier><identifier>DOI: 10.1007/BF00210350</identifier><identifier>PMID: 7020949</identifier><language>eng</language><publisher>Germany</publisher><subject>acetylchlorine ; Acetylcholinesterase - metabolism ; Animals ; antisera ; Complement Fixation Tests ; Diaphragm - innervation ; Electric Organ - innervation ; Fishes ; Fluorescent Antibody Technique ; Ganglia, Sympathetic - ultrastructure ; Guinea Pigs ; Hippocampus - ultrastructure ; Ileum - innervation ; Parasympathetic Nervous System - ultrastructure ; Rats ; synaptosomes ; Synaptosomes - ultrastructure ; Torpedo marmorata</subject><ispartof>Cell and tissue research, 1981-01, Vol.218 (2), p.355-373</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c313t-a34430dd4f67927e3bdae3d3fe52188edf6b727e31b1ea92cf31d4674384cb7b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7020949$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jones, R T</creatorcontrib><creatorcontrib>Walker, J H</creatorcontrib><creatorcontrib>Richardson, P J</creatorcontrib><creatorcontrib>Fox, G Q</creatorcontrib><creatorcontrib>Whittaker, V P</creatorcontrib><title>Immunohistochemical localization of cholinergic nerve terminals</title><title>Cell and tissue research</title><addtitle>Cell Tissue Res</addtitle><description>Most of the published light-microscopic methods for the localization of cholinergic nerve pathways present various difficulties of interpretation. The production and characterization of an antiserum that binds specifically to cholinergic terminals is described. The antiserum was raised to small synaptosomes prepared from the purely cholinergic electric organ of Torpedo marmorata. It was shown to lyse cholinergic synaptosomes in a mixed population derived from guinea-pig cortex. After partial purification by adsorption onto nonspecific antigens, It was used to label nerve endings in several tissues of Torpedo, rat and guinea pigs using indirect immunofluorescence histochemistry. 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The production and characterization of an antiserum that binds specifically to cholinergic terminals is described. The antiserum was raised to small synaptosomes prepared from the purely cholinergic electric organ of Torpedo marmorata. It was shown to lyse cholinergic synaptosomes in a mixed population derived from guinea-pig cortex. After partial purification by adsorption onto nonspecific antigens, It was used to label nerve endings in several tissues of Torpedo, rat and guinea pigs using indirect immunofluorescence histochemistry. The antiserum appears to provide a highly specific means of localizing cholinergic nerve endings in these tissues.</abstract><cop>Germany</cop><pmid>7020949</pmid><doi>10.1007/BF00210350</doi><tpages>19</tpages></addata></record>
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source	Springer LINK Archives
subjects	acetylchlorine Acetylcholinesterase - metabolism Animals antisera Complement Fixation Tests Diaphragm - innervation Electric Organ - innervation Fishes Fluorescent Antibody Technique Ganglia, Sympathetic - ultrastructure Guinea Pigs Hippocampus - ultrastructure Ileum - innervation Parasympathetic Nervous System - ultrastructure Rats synaptosomes Synaptosomes - ultrastructure Torpedo marmorata
title	Immunohistochemical localization of cholinergic nerve terminals
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