Establishment and characterization of a spontaneously immortalized mouse ameloblast-lineage cell line

Tooth development was cooperatively regulated by the epithelial ameloblasts and mesenchymal odontoblasts. Ameloblasts secrete enamel matrix, critical for enamel formation. While there are several reports about establishment of immortalized ameloblast-like cells by introducing viral oncogene, we trie...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 2003-09, Vol.308 (4), p.834-839
Main Authors: Nakata, Akira, Kameda, Takashi, Nagai, Hirokazu, Ikegami, Kenji, Duan, Yuqing, Terada, Kunihiko, Sugiyama, Toshihiro
Format: Article
Language:English
Subjects:
Alkaline Phosphatase - metabolism
Ameloblast
Ameloblasts - cytology
Amelogenin
Animals
Animals, Newborn
Calcification
Calcium - metabolism
Cell Line
Cell Lineage
Cells, Cultured
Dental Enamel Proteins - metabolism
DNA, Complementary - metabolism
Enamel matrix
Epithelial Cells - metabolism
Gene Expression Regulation
In Situ Hybridization
Mice
Mice, Inbred C57BL
Mice, Nude
Reverse Transcriptase Polymerase Chain Reaction
Spontaneous immortalization
Tooth - cytology
Transforming Growth Factor beta - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Tooth development was cooperatively regulated by the epithelial ameloblasts and mesenchymal odontoblasts. Ameloblasts secrete enamel matrix, critical for enamel formation. While there are several reports about establishment of immortalized ameloblast-like cells by introducing viral oncogene, we tried to establish a spontaneously immortalized ameloblast-lineage cell line, maintaining the cell type specific character, including the ability to induce in vitro bio-mineralization. The established cell line (ameloblast-lineage cell; ALC) maintained the expression of several ameloblast specific genes ( Amelogenin, Tuftelin, and Enamelin) in long-term culture. They formed calcified nodules after the induction by medium switching from SMEM to DMEM, having high-level alkaline-phosphatase activity. The size and number of calcified nodule formation were enhanced by TGF-β treatment. Six weeks after sub-cutaneous implantation of ALC to athymic nude mice, we ectopically observed enamel epithelium like structure formation, chondrogenesis, and calcification. These data indicate that ALC is a useful experimental tool to analyze ameloblast character.
ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(03)01467-0