Surface film pressure of actin: interactions with lipids in mixed monolayers

The interactions of actin with neutral lipid films made from DLPC, and with positively charged films built from DLPC and stearylamine (SA), have been characterized by the monolayer technique. Injection of actin underneath an expanded lipid film produces an increase in the surface pressure that is co...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2003-09, Vol.308 (4), p.995-1000
Main Authors: Gicquaud, C, Chauvet, J.-P, Tancrède, P
Format: Article
Language:English
Subjects:
Actin
Actins - chemistry
Actins - metabolism
Actin–lipid interactions
Amines - chemistry
Animals
Biophysical Phenomena
Biophysics
Lipid Metabolism
Lipids - chemistry
Magnesium - chemistry
Magnesium - metabolism
Models, Chemical
Monolayer
Muscle, Skeletal - metabolism
Phosphatidylcholines - chemistry
Pressure
Rabbits
Surface tension
Temperature
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Summary:The interactions of actin with neutral lipid films made from DLPC, and with positively charged films built from DLPC and stearylamine (SA), have been characterized by the monolayer technique. Injection of actin underneath an expanded lipid film produces an increase in the surface pressure that is consistent with a penetration of the lipid molecules by actin. This adsorption of actin to the lipid is more pronounced either with positively charged films or with Mg 2+ present in the sub-phase, suggesting that the mechanism involves an electrostatic attraction. During compression, the actin molecules are squeezed out into the sub-phase, carrying along some lipid molecules; this suggests a strong affinity of the lipids for actin. An analysis of the dilational modulus shows that when actin is found as monomers at the interface, the mixed actin–lipid film undergoes three phase changes upon compression. On the other hand, when actin is polymerized at the interface, the actin and the lipid form a rigid film for which the compressibility is mostly dominated by actin.
ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(03)01505-5