DNA damage in digestive gland and mantle tissue of the mussel Perna perna

• Published in: Comparative biochemistry and physiology. Toxicology & pharmacology 2003-07, Vol.135 (3), p.295-303
• Main Authors: de Almeida, Eduardo Alves, de Almeida Marques, Sabrina, Klitzke, Clécio Fernando, Bainy, Afonso Celso Dias, de Medeiros, Marisa Helena Gennari, Di Mascio, Paolo, de Melo Loureiro, Ana Paula
• Format: Article
• Language: English
• Subjects: 8-oxodGuo; Animals; Bivalvia; Bivalvia - chemistry; Bivalvia - metabolism; DNA damage; DNA Damage - physiology; Etheno adduct; Gastrointestinal Tract - chemistry; Gastrointestinal Tract - metabolism; HPLC; Lipid peroxidation; Marine; Mass spectrometry; Mussel; Oxidative stress; Oxidative Stress - physiology; Perna perna; Reactive Oxygen Species - analysis; Reactive Oxygen Species - metabolism
• ISSN: 1532-0456; 1878-1659
• DOI: 10.1016/S1532-0456(03)00117-0

Data concerning the susceptibility of DNA to damage by reactive oxygen and nitrogen species and other endogenous compounds produced by physiological stress in marine organisms is lacking, especially in bivalve mollusks. In this article, we analyzed the background levels of lipid peroxidation (malondialdehyde, MDA), 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodGuo) and 1, N 2-etheno-2′-deoxyguanosine (1, N 2-εdGuo) in digestive gland and mantle tissue of mussels Perna perna collected at a cultivation zone in Florianópolis (Santa Catarina, Brazil). The present data point to the possibility of the use of both 8-oxodGuo and 1, N 2-εdGuo as complementary indicators of oxidative stress processes in mussels. A sensitive method coupling high performance liquid chromatography to mass spectrometry was applied for the detection of 1, N 2-εdGuo in mussel tissues.