Separation of mercury substituted RNA synthesized in isolated rat liver nuclei

The population of RNA molecules synthesized in isolated rat liver nuclei in vitro in the presence of [3H]CTP and Hg-UTP was successfully fractionated into at least two subfractions containing various proportions of mercury label. Fractionation was achieved either by step-wise chromatography of Hg-RN...

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Bibliographic Details
Published in:Molecular biology reports 1981-01, Vol.7 (1-3), p.57-62
Main Authors: Hanausek-Walaszek, M, Walaszek, Z, Chorazy, M
Format: Article
Language:English
Subjects:
Animals
Cell Nucleus - metabolism
Centrifugation, Density Gradient
Chromatography, Gel
Cytidine Triphosphate - metabolism
Liver - metabolism
Mercury - metabolism
Metrizamide
Rats
RNA - metabolism
Transcription, Genetic
Uridine Triphosphate - metabolism
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Summary:The population of RNA molecules synthesized in isolated rat liver nuclei in vitro in the presence of [3H]CTP and Hg-UTP was successfully fractionated into at least two subfractions containing various proportions of mercury label. Fractionation was achieved either by step-wise chromatography of Hg-RNA on thiopropyl-Sepharose columns or by density gradient centrifugation in metrizamide. The fraction of RNA heavily labeled with Hg-UTP was composed mainly of 4--18S RNA and contained virtually all radioactivity derived from [gamma-32P]ATP or [gamma-32P]GTP. The slightly mercurated RNA fraction consisted mainly of longer RNA molecules (12- greater than 28S) and was not labeled with [gamma-32P]ATP or [gamma-32P]GTP. Labeling with gamma-32P nucleoside triphosphates was sensitive both to rifamycin AF/013 and heparin whereas labeling with [3H]CTP was fully resistant to the inhibitors and showed sensitivity to low doses of alpha-amanitin. We assume that the observed subpopulation of heavily mercurated RNAs consists of RNA molecules initiated in vitro.
ISSN:0301-4851
1573-4978
DOI:10.1007/BF00778734