Effect of siliconized latex urinary catheters on the activity of carbapenems against Pseudomonas aeruginosa strains with defined mutations in ampC, oprD, and genes coding for efflux systems

The decreased activity of carbapenems against Pseudomonas aeruginosa in the presence of eluates from siliconized latex urinary catheters is related to the loss of OprD and the expression of a new outer membrane protein (OMP). To understand this phenomenon, the activity of carbapenems and quinolones...

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Bibliographic Details
Published in:International journal of antimicrobial agents 2003-08, Vol.22 (2), p.122-127
Main Authors: Carmen Conejo, M., Martı&#x0301, nez-Martı&#x0301, nez, Luis, Garcı&#x0301, a, Isabel, Picabea, Leandro, Pascual, Álvaro
Format: Article
Language:English
Subjects:
ampC
Anti-Infective Agents - pharmacology
Antibacterial agents
Antibiotics. Antiinfectious agents. Antiparasitic agents
Bacterial Proteins
beta-Lactamases - genetics
Biofilms
Biological and medical sciences
Carbapenems - pharmacology
Drug Resistance, Bacterial - genetics
Efflux systems
Genes, Bacterial
Humans
In Vitro Techniques
Latex
Medical sciences
Microbial Sensitivity Tests
Mutation
oprD
Pharmacology. Drug treatments
Porins - genetics
Pseudomonas aeruginosa - drug effects
Pseudomonas aeruginosa - genetics
Pseudomonas aeruginosa - isolation & purification
Pseudomonas Infections - drug therapy
Pseudomonas Infections - microbiology
Silicones
Urinary Catheterization - adverse effects
Urinary Catheterization - instrumentation
Urinary Tract Infections - drug therapy
Urinary Tract Infections - microbiology
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Summary:The decreased activity of carbapenems against Pseudomonas aeruginosa in the presence of eluates from siliconized latex urinary catheters is related to the loss of OprD and the expression of a new outer membrane protein (OMP). To understand this phenomenon, the activity of carbapenems and quinolones against P. aeruginosa strains, some producing AmpC β-lactamase (BL), OprD, MexA–MexB–OprM and MexE–MexF–OprN, were determined in Mueller–Hinton broth (MH) and in MH-containing eluates. OMP profiles and BL activities were also studied. Resistance to imipenem in P. aeruginosa grown in eluate is due to both the loss of OprD and expression of BL. For meropenem this phenomenon is related to both the loss of OprD and the integrity of MexA–MexB–OprM.
ISSN:0924-8579
1872-7913
DOI:10.1016/S0924-8579(03)00119-5