Immunity to schistosomiasis: glycans are potential antigenic targets for immune intervention

• Published in: Experimental parasitology 2003-05, Vol.104 (1), p.1-13
• Main Authors: Nyame, A.Kwame, Lewis, Fred A., Doughty, Barbara L., Correa-Oliveira, Rodrigo, Cummings, Richard D.
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Helminth - biosynthesis; Antibodies, Helminth - blood; Antibodies, Helminth - immunology; Antibodies, Monoclonal - immunology; Antigens, Helminth - chemistry; Antigens, Helminth - immunology; Blotting, Western; Carbohydrate Sequence; Complement System Proteins - immunology; Enzyme-Linked Immunosorbent Assay; Feces - parasitology; Female; Flow Cytometry; Humans; Immunoglobulins - biosynthesis; Immunoglobulins - blood; Immunohistochemistry; Male; Mice; Microscopy, Confocal; Molecular Sequence Data; Polysaccharides - chemistry; Polysaccharides - immunology; Schistosoma mansoni; Schistosoma mansoni - immunology; Schistosomiasis mansoni - immunology; Schistosomiasis mansoni - parasitology
• ISSN: 0014-4894; 1090-2449
• DOI: 10.1016/S0014-4894(03)00110-3

The major humoral immune responses in animals infected with Schistosoma mansoni are directed toward carbohydrate antigens. Among these antigens are complex-type N-glycans expressing LDN [GalNAcβ1–4GlcNAc-R], LDNF [GalNAcβ1–4(Fucα1–3)GlcNAc-R], and polymeric Lewis x (Le x) [Galβ1–4(Fucα1–3)GlcNAc] n -R epitopes. We have now evaluated the potential of the three glycan antigens as targets for immune-mediated intervention of infections and serodiagnosis. A variety of approaches were employed, including ELISA, Western blot, immunohistology, and in vitro complement lysis assays, to determine the immunogenicity of the glycans in infected humans, their localization on the parasites and their efficacy as targets for parasite lysis. Our results show that S. mansoni-infected patients, with either intestinal or hepatosplenic disease, generate predominantly IgM, but also IgG and IgA, antibodies to LDN, LDNF, and Le x. However, immune responses to Le x are generally lower than responses to LDN and LDNF and less specific to schistosome infections. Western blot analysis with monoclonal antibodies (mAb) to LDN, LDNF, and Le x determinants show that the glycan antigens occur on multiple glycoproteins from cercariae, 3-h, 48-h, and lung stage schistosomula, as well as adults and eggs. Immunohistological studies demonstrate that LDN, LDNF, and Le x are expressed on the parasite surface at all stages of development in the vertebrate host. Importantly, a mAb to LDN in the presence of complement efficiently kills schistosomula in vitro, as demonstrated by flow-cytometric assays that quantify cytolysis by propidium iodide uptake into damaged parasites. These findings raise the possibility that LDN and LDNF may be targets for vaccination and/or serodiagnosis of chronic schistosomiasis in humans. Abbreviations used: LDN—LacdiNAc, GalNAcβ1 → 4GlcNAc; LDNF, fucosylated lacdiNAc, GalNAcβ1 → 4(Fucα1 → 3)GlcNAc; Le x—Lewis x, Galβ1 → 4(Fucα1 → 3)GlcNAc; LDNT—lacdiNActetraose, GalNAcβ1 → 4GlcNAcβ1 → 3Galβ1 → 4GlcNAc; LDNFP—lacdiNAc fucopentaose GalNAcβ 1 → 4(Fucα1 → 3)GlcNAcβ1 → 3Galβ1 → 4GlcNAc; LNFPIII—lacto- N-fucopentaose III, Galβ1 → 4(Fucα1 → 3)GlcNAcβ1 → 3Galβ1 → 4GlcNAc; SEA, soluble egg antigen; PBS, phosphate-buffered saline; BSA, bovine serum albumin; BCA, bicinchoninic acid; SDS–PAGE, sodium dodecyl sulfate–polyacrylamide gel electrophoresis; ASGP-R, asialoglycoprotein receptor