Characterization of NADE, NRIF and SC-1 gene expression during mouse neurogenesis

• Published in: Brain research. Developmental brain research 2003-09, Vol.144 (2), p.151-158
• Main Authors: Kendall, Stephen E., Ryczko, Michael C., Mehan, Mala, Verdi, Joseph M.
• Format: Article
• Language: English
• Subjects: Aging; Animals; Animals, Newborn; Apoptosis; Apoptosis Regulatory Proteins; Carrier Proteins - biosynthesis; Carrier Proteins - genetics; Cerebral Cortex - cytology; Cerebral Cortex - embryology; Cerebral Cortex - growth & development; Cerebral Cortex - metabolism; Embryo, Mammalian; Female; Gene Expression; Gene Expression Regulation, Developmental; In Situ Hybridization; Intracellular Signaling Peptides and Proteins; Male; Mice; NADE; Neurons - metabolism; Neurotrophin; NRIF; p75 NTR; Pregnancy; Protein Biosynthesis; Proteins - genetics; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - metabolism; SC-1; Spinal Cord - cytology; Spinal Cord - embryology; Spinal Cord - growth & development; Spinal Cord - metabolism; Tissue Distribution
• ISSN: 0165-3806
• DOI: 10.1016/S0165-3806(03)00166-4

The p75 neurotrophin receptor (p75 NTR) is a member of the tumor necrosis factor receptor superfamily. p75 NTR signaling events have been implicated in both cell cycle arrest and apoptosis depending on which effector molecules are associated with its intracellular domain after ligand binding. Two such effector proteins, p75 NTR-associated cell death executor (NADE) and neurotrophin receptor interacting factor (NRIF) promote p75 NTR-mediated apoptosis, whereas Schwann cell factor-1 (SC-1) mediates neurotrophin-dependent withdrawal from the cell cycle. An understanding of the expression profiles of these three interacting proteins and p75 NTR during embryogenesis is critical for addressing whether these effector proteins might function outside of p75 NTR-mediated signaling events. The distribution of NADE, NRIF and SC-1 mRNAs during murine development suggests that the action of these genes is in fact not limited to regions of p75 NTR expression. Specifically, a detailed comparison of the spatial and temporal expression domains of NADE, NRIF and SC-1 during brain development revealed regions of co-expression with p75 NTR but also illustrates a distinct and discordant spatial and temporal expression. These results yield novel insights into the unique developmental characteristics of the three p75 NTR-interacting proteins, thus revealing their diverse signaling potential during embryonic development.