p27 Labeling Index and Proliferation in Gastrointestinal Stromal Tumors: Correlations with Clinicopathologic Factors and Recurrence

• Published in: Japanese journal of clinical oncology 2003-07, Vol.33 (7), p.346-352
• Main Authors: Gelen, Tekinalp, Elpek, Gülsüm Özlem, Aksoy, Nazif Hikmet, Ögüs, Mehmet, Keles, Nuran
• Format: Article
• Language: English
• Subjects: Adult; Cell Cycle Proteins - biosynthesis; Cyclin-Dependent Kinase Inhibitor p27; Disease-Free Survival; Female; Gastrointestinal Neoplasms - metabolism; Gastrointestinal Neoplasms - pathology; Gastrointestinal Neoplasms - surgery; Humans; Immunohistochemistry; Key words: p27 – proliferation – Ki-67 – gastrointestinal stromal tumors – recurrence; Ki-67 Antigen - biosynthesis; Male; Middle Aged; Mitotic Index; Neoplasm Recurrence, Local - epidemiology; Prognosis; Stomach Neoplasms - metabolism; Stomach Neoplasms - pathology; Stomach Neoplasms - surgery; Survival Analysis; Tumor Suppressor Proteins - biosynthesis
• ISSN: 0368-2811; 1465-3621; 1465-3621
• DOI: 10.1093/jjco/hyg071

Background: Low expression of p27, a cyclin-dependent kinase inhibitor, has recently been reported to be associated with poor prognosis of many tumors. Moreover, an inverse relationship between p27 expression and proliferation was also noted. Although accumulating data indicate a correlation between high proliferation rate and aggressive behavior of gastrointestinal stromal tumors, no conclusive correlation between p27 expression and either tumor recurrence or proliferation has been established yet in this disease. The aim of this study was to immunohistochemically investigate the association of p27 expression (as measured by the p27 labeling index: p27LI) with recurrence and proliferation (as measured by the Ki-67 labeling index: KLI) in gastrointestinal stromal tumors. Methods: p27LI and KLI were investigated in tumor specimens from 50 patients diagnosed with gastrointestinal stromal tumors. Quantitative evaluations of p27LI and KLI were performed on tissue sections stained immunohistochemically with anti-p27 and anti-Ki-67 monoclonal antibodies. Results: Both p27LI and KLI were associated with the presence of recurrence and high mitotic index. A significant inverse correlation was noted between p27LI and KLI (r = –0.82). The recurrence-free survival time was significantly shorter in patients with high KLI (≥10%), low p27LI (