Adsorptive Stripping Voltammetric Determination of Cefepime at the Mercury Electrode in Human Urine and Cerebrospinal Fluid, and Differential Pulse Polarographic Determination in Serum

The cephalosporin cefepime has been studied by adsorptive stripping voltammetric on the hanging mercury drop electrode, followed by linear sweep voltammetry (staircase). The adsorptive stripping response was evaluated with respect to preconcentration dependence and other variables. The drug is stron...

Full description

Saved in:
Bibliographic Details
Published in:Journal of pharmaceutical sciences 2003-09, Vol.92 (9), p.1854-1859
Main Authors: Palacios, F.J.Jiménez, Mochón, M.Callejón, Sánchez, J.C.Jiménez, Carranza, J.Herrera
Format: Article
Language:English
Subjects:
adsorptive stripping voltammetry
Analysis
Anti-Bacterial Agents - analysis
Anti-Bacterial Agents - blood
Anti-Bacterial Agents - cerebrospinal fluid
Anti-Bacterial Agents - urine
Biological and medical sciences
cefepime determination
Cephalosporins - analysis
Cephalosporins - blood
Cephalosporins - cerebrospinal fluid
Cephalosporins - urine
cerebrospinal fluid
Electrochemistry - instrumentation
Electrochemistry - methods
Electrodes
General pharmacology
Humans
Medical sciences
Mercury
Pharmacology. Drug treatments
Polarography - methods
Serum
urine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The cephalosporin cefepime has been studied by adsorptive stripping voltammetric on the hanging mercury drop electrode, followed by linear sweep voltammetry (staircase). The adsorptive stripping response was evaluated with respect to preconcentration dependence and other variables. The drug is strongly adsorbed in acid media, with maximum adsorption at pH 5.8. The detection limit found was 4.8×10−10 M, with 120-s preconcentration. The relative standard deviation at the 10−7 M level was 0.93%. This method was applied to the determination of cefepime in human urine and cerebrospinal fluid. Differential pulse polarography has been applied to determination in human serum. © 2003 Wiley-Liss, Inc. and the American Pharmacists Association.
ISSN:0022-3549
1520-6017
DOI:10.1002/jps.10438