The Chamber/Dish: An Improved Vessel for Cell and Explant Culture

A vessel consisting of a Teflon well former, metal base plate, rubber gasket, and variable growth surface has been constructed. It is nontoxic, autoclavable, and reusable. It provides for ready access into the growth chamber, plus an optically clear lower surface for improved microscopic resolution....

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Bibliographic Details
Published in:In Vitro 1981-02, Vol.17 (2), p.91-97
Main Author: Gabridge, Michael G.
Format: Article
Language:English
Subjects:
Animals
Bacteriological Techniques
Bottles
Cell culture techniques
Cell Division
Cell growth
Cell Line
Cells, Cultured - cytology
Cricetinae
Culture Techniques - instrumentation
Cytological Techniques - instrumentation
Fibroblasts
Gaskets
Humans
Lungs
Magnification
Microscopy
Mycoplasma pneumoniae - growth & development
Rubber
Vero cells
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Summary:A vessel consisting of a Teflon well former, metal base plate, rubber gasket, and variable growth surface has been constructed. It is nontoxic, autoclavable, and reusable. It provides for ready access into the growth chamber, plus an optically clear lower surface for improved microscopic resolution. The vessel has been used successfully with several different eucaryotic and procaryotic cell systems. Morphology of cells and yields (as measured by total protein and ATP) are comparable to those noted with other vessels. This Chamber/Dish can accommodate almost any type of growth substrate in the form of films or sheets. It appears to have widespread application for the growth of cells and explants in vitro.
ISSN:0073-5655
1475-2689
DOI:10.1007/BF02618064