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Immunochemical evidence of homologies among 50 S ribosomal proteins of Bacillus stearothermophilus and Escherichia coli
Antibodies prepared against individual 50 S ribosomal subunit proteins from Escherichia coli were reacted with 70 S ribosomal proteins from Bacillus stearothermophilus in order to identify homologous protein pairs. B. stearothermophilus proteins were separated by two-dimensional polyacrylamide gel e...
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Published in: | The Journal of biological chemistry 1981-10, Vol.256 (19), p.10111-10116 |
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Main Authors: | , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Antibodies prepared against individual 50 S ribosomal subunit proteins from Escherichia coli were reacted with 70 S ribosomal
proteins from Bacillus stearothermophilus in order to identify homologous protein pairs. B. stearothermophilus proteins were
separated by two-dimensional polyacrylamide gel electrophoresis and transferred electrophoretically to diazobenzyloxymethyl
paper to which they became covalently attached. The paper was then washed with antiserum followed by radioactive protein A,
and the resulting antigen-antibody-protein A complexes were located by autoradiography. Seventeen cross-reacting protein pairs
were identified. |
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ISSN: | 0021-9258 1083-351X |
DOI: | 10.1016/S0021-9258(19)68750-8 |