Phenoloxidase activity in larval and juvenile homogenates and adult plasma and haemocytes of bivalve molluscs

Phenoloxidase (PO) activity was studied in larval and juvenile homogenates and in the plasma and haemocytes of adult Crassostrea gigas, Argopecten ventricosus, Nodipecten subnodosus, and Atrina maura. Samples were tested for the presence of PO activity by incubation with the substrate l-3, 4-dihydro...

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Bibliographic Details
Published in:Fish & shellfish immunology 2003-10, Vol.15 (4), p.275-282
Main Authors: Luna-González, Antonio, Maeda-Martı́nez, Alfonso N, Vargas-Albores, Francisco, Ascencio-Valle, Felipe, Robles-Mungaray, Miguel
Format: Article
Language:English
Subjects:
Analysis of Variance
Animals
Argopecten ventricosus
Atrina maura
Catechol Oxidase - metabolism
Chymotrypsin - metabolism
Crassostrea gigas
Enzyme Precursors - metabolism
Haemocytes
Hemocytes - enzymology
Immunology
Juveniles
Larvae
Levodopa - metabolism
Lipopolysaccharides - metabolism
Marine
Mollusca - enzymology
Mollusca - immunology
Monophenol Monooxygenase - blood
Monophenol Monooxygenase - metabolism
Nodipecten subnodosus
Prophenoloxidase
Protease Inhibitors - metabolism
Sodium Dodecyl Sulfate - metabolism
Spectrophotometry
Trypsin - metabolism
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Summary:Phenoloxidase (PO) activity was studied in larval and juvenile homogenates and in the plasma and haemocytes of adult Crassostrea gigas, Argopecten ventricosus, Nodipecten subnodosus, and Atrina maura. Samples were tested for the presence of PO activity by incubation with the substrate l-3, 4-dihydroxyphenylalanine using trypsin, α-chymotrypsin, laminarin, lipopolysaccharides (LPS), and sodium dodecyl sulphate (SDS) to elicit activation of prophenoloxidase (proPO) system. PO activity was not detected in larval homogenate. In juvenile homogenate, PO activity was found only in C. gigas and N. subnodosus. PO activity was present in adult samples and was enhanced by elicitors in the plasma of all species tested, but in haemocyte lysate supernatant (HLS) of only N. subnodosus. Activation of proPO by laminarin was suppressed by a protease inhibitor cocktail (P-2714) in plasma and HLS of all species tested.
ISSN:1050-4648
1095-9947
DOI:10.1016/S1050-4648(02)00165-1