Corticotropin-releasing hormone deficiency results in impaired splenocyte response to lipopolysaccharide

Corticotropin-releasing hormone (Crh), a major mediator of the stress response, has been shown to exert both stimulatory and inhibitory effects on the regulation of the immune system, in vivo. In our present study, we used the Crh−/− mice to investigate the effect of Crh deficiency on leukocyte func...

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Bibliographic Details
Published in:Journal of neuroimmunology 2003-08, Vol.141 (1), p.3-9
Main Authors: Venihaki, Maria, Zhao, Jie, Karalis, Katia P
Format: Article
Language:English
Subjects:
Animals
Cell Division - genetics
Cell Division - immunology
Cells, Cultured
Corticotropin-Releasing Hormone - biosynthesis
Corticotropin-Releasing Hormone - deficiency
Corticotropin-Releasing Hormone - pharmacology
Corticotropin-Releasing Hormone - physiology
CRH
Cytokine
Cytokines - biosynthesis
Cytokines - genetics
Cytokines - metabolism
Down-Regulation - genetics
Down-Regulation - immunology
Interleukin-1 - metabolism
Lipopolysaccharides - pharmacology
Lymphocyte Activation - genetics
Lymphocyte Subsets - immunology
Lymphocyte Subsets - metabolism
Lymphocyte Subsets - pathology
Mice
Mice, Inbred C57BL
Mice, Knockout
NF-kappa B - antagonists & inhibitors
NF-kappa B - metabolism
Proliferation
RNA, Messenger - antagonists & inhibitors
RNA, Messenger - biosynthesis
Spleen - drug effects
Spleen - immunology
Spleen - metabolism
Spleen - pathology
Splenocyte
Tumor Necrosis Factor-alpha - metabolism
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Summary:Corticotropin-releasing hormone (Crh), a major mediator of the stress response, has been shown to exert both stimulatory and inhibitory effects on the regulation of the immune system, in vivo. In our present study, we used the Crh−/− mice to investigate the effect of Crh deficiency on leukocyte function in vitro. Our results show that following LPS treatment, TNF-α and IL-1β expression was significantly compromised in Crh−/− splenocytes, an effect most likely mediated by the lower levels of NF-κB DNA binding activity measured in the same cells. Furthermore, we show here that the proliferation rate of Crh−/− splenocytes in response to LPS was decreased compared to Crh+/+ splenocytes. Taken together, our findings show that the presence of endogenous Crh is necessary for the normal function of leukocytes, in vitro.
ISSN:0165-5728
1872-8421
DOI:10.1016/S0165-5728(03)00183-8