Cloning of a representative genomic library of the human X chromosome after sorting by flow cytometry

A library of 50,000 recombinants representative of the human X chromosome has been constructed. Human X chromosomes were physically separated using a fluorescence-activated cell sorter. The DNA was purified from the chromosomes, digested to completion with the restriction enzyme Eco RI and cloned in...

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Bibliographic Details
Published in:Nature (London) 1981-10, Vol.293 (5831), p.374-376
Main Authors: Davies, Kay E, Young, Bryan D, Elles, Robert G, Hill, Marion E, Williamson, Robert
Format: Article
Language:English
Subjects:
Bacteriophage lambda - genetics
Cell Line
Cell Separation - methods
Cloning, Molecular - methods
DNA, Recombinant
Female
Flow Cytometry - methods
Humanities and Social Sciences
Humans
multidisciplinary
Science
Science (multidisciplinary)
Sex Chromosomes
X Chromosome
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Summary:A library of 50,000 recombinants representative of the human X chromosome has been constructed. Human X chromosomes were physically separated using a fluorescence-activated cell sorter. The DNA was purified from the chromosomes, digested to completion with the restriction enzyme Eco RI and cloned into the phage λgtWES.λB. The X-derived nature of the recombinants was confirmed by hybridization to rodent/human cell line DNA containing only the human X chromosome. Such libraries will be particularly useful for the investigation of genetic diseases such as Duchenne muscular dystrophy, where the basic defect has not been elucidated, and of neoplasia, where several specific chromosomal anomalies, particularly for the leukaemias, have been identified.
ISSN:0028-0836
1476-4687
DOI:10.1038/293374a0