Study on interaction between T4 phage and Escherichia coli B by microcalorimetric method

• Published in: Journal of virological methods 2003-09, Vol.112 (1), p.137-143
• Main Authors: Guosheng, Liu, Yi, Liu, Xiangdong, Chen, Peng, Liu, Ping, Shen, Songsheng, Qu
• Format: Article
• Language: English
• Subjects: Bacteriological methods and techniques used in bacteriology; Bacteriology; Bacteriolysis; Bacteriophage T4 - growth & development; Bacteriophage T4 - physiology; Biological and medical sciences; Calorimetry - instrumentation; Calorimetry - methods; Escherichia coli - cytology; Escherichia coli - metabolism; Escherichia coli - virology; Escherichia coli B; Fundamental and applied biological sciences. Psychology; Hot Temperature; Kinetics; Microbiology; Microcalorimetry; Nephelometry and Turbidimetry - methods; Phage–host interaction; T4 phage; Thermokinetics
• ISSN: 0166-0934; 1879-0984
• DOI: 10.1016/S0166-0934(03)00214-3

The process that T4 phages multiply in host cells of Escherichia coli B was determined using LKB-2277 Bioactivity Monitor by means of stopped-flow method, and the growth was measured turbidometrically at the same time at 37 °C. By analyzing thermo-curves, quantitative parameters could be obtained to characterize the interactions of host cells and phages. The parameters such as k a , P max , G etc. change regularly with the decrease of multiplicity of infection (MOI) value. Infection–lysis equations were fitted and the lytic rate constant k L was obtained. The results show that the metabolic activity of infected cells is more intensive than that of normal cells. The phenomenon of lysis inhibition (LIN) was first detected with the microcalorimetric method, and the mechanism is discussed.