Cloning and embryonic expression pattern of the mouse Onecut transcription factor OC-2

Onecut (OC) transcription factors are evolutionarily conserved proteins with important developmental functions. They contain a bipartite DNA-binding domain composed of a single cut domain associated with a divergent homeodomain. The human genome contains three Onecut paralogues, Hnf6 (also called Oc...

Full description

Saved in:
Bibliographic Details
Published in:Gene Expression Patterns 2003-10, Vol.3 (5), p.639-644
Main Authors: Jacquemin, Patrick, Pierreux, Christophe E., Fierens, Sébastien, van Eyll, Jonathan M., Lemaigre, Frédéric P., Rousseau, Guy G.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Cloning, Molecular
Conserved Sequence
Duodenum
Endoderm
Gene Expression Regulation, Developmental
Hnf6
Homeodomain Proteins - genetics
Liver
Liver - embryology
Liver - metabolism
Mice
Molecular Sequence Data
Mouse
Nervous system
Nervous System - embryology
Nervous System - metabolism
Oc2
Onecut
Pancreas
Pancreas - embryology
Pancreas - metabolism
Sequence Alignment
Sequence Homology, Amino Acid
Transcription factors
Transcription Factors - genetics
Transcription Factors - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Onecut (OC) transcription factors are evolutionarily conserved proteins with important developmental functions. They contain a bipartite DNA-binding domain composed of a single cut domain associated with a divergent homeodomain. The human genome contains three Onecut paralogues, Hnf6 (also called Oc1), Oc2 and Oc3. We describe here the cloning of mouse (m) OC-2 and its expression pattern in the mouse embryo. The m Oc2 gene was localized on chromosome 18. Analysis of the mOC-2 amino acid sequence revealed overall identities of 67% with mHNF-6 and of 56% with mOC-3, and the presence of functional domains delineated earlier in HNF-6. The sequence of the 153 residue-long cut-homeodomain was very conserved, as it was 92% identical to that of mHNF-6 and 89% identical to that of mOC-3. In situ hybridization showed expression of m Oc2 in the developing nervous system and gut endoderm. Like Hnf6, Oc2 was expressed in developing liver and pancreas. As many genes that are targeted by Onecut factors are recognized by both OC-2 and HNF-6, this overlap of expression patterns may have functional implications.
ISSN:1567-133X
1872-7298
DOI:10.1016/S1567-133X(03)00110-8