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Gene expression of type 2 17β hydroxysteroid dehydrogenase in scalp hairs of hirsute women
Androgens are the main hormonal regulators of human hair growth and they are related to clinical conditions such as hirsutism. The aim of this study was to analyze the gene expression of androgen receptor (AR) and type 2 17β hydroxysteroid dehydrogenase (17β-HSD) in keratinocytes of plucked scalp ha...
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Published in: | Steroids 2003-09, Vol.68 (7), p.641-649 |
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description | Androgens are the main hormonal regulators of human hair growth and they are related to clinical conditions such as hirsutism. The aim of this study was to analyze the gene expression of androgen receptor (AR) and type 2 17β hydroxysteroid dehydrogenase (17β-HSD) in keratinocytes of plucked scalp hairs from hirsute patients and normal subjects. We studied 58 women with hirsutism (31 with polycystic ovary syndrome (PCOS), 27 with idiopathic hirsutism (IH)); 15 control women; and 10 control men. Hirsutism was assessed by a modified Ferriman–Gallwey method. Hormonal status was assessed between days 2 and 10 of the menstrual cycle or on any day when the patients were amenorrheic. AR and type 2 17β-HSD mRNA levels were estimated by reverse transcription-polymerase chain reaction (RT-PCR). AR expression was similar in all groups. Type 2 17β-HSD gene expression in untreated hirsute patients was lower (2.1±0.10) than in normal women (3.1±0.17), and similar to men (1.8±0.22). Comparing hirsute patients, type 2 17β-HSD expression was higher in treated PCOS (3.0±0.34 versus 2.2±0.13) and IH patients (2.5±0.19 versus 2.0±0.15); hirsutism score was lower (
P=0.003, PCOS;
P=0.003, IH); and SHBG levels were higher (
P=0.001, PCOS;
P=0.024, IH) in treated patients. The free androgen index was lower in treated women (
P=0.024 for the IH group). In conclusion, the lower expression of type 2 17β-HSD mRNA in scalp hairs of untreated hirsute patients suggests androgen metabolism disturbances with predominance of more potent androgens, as occurs in men. The enzyme’s higher gene expression in treated hirsute patients could be an indirect evidence of restored enzyme activity and intracellular androgen metabolism. |
doi_str_mv | 10.1016/S0039-128X(03)00093-X |
format | article |
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P=0.003, PCOS;
P=0.003, IH); and SHBG levels were higher (
P=0.001, PCOS;
P=0.024, IH) in treated patients. The free androgen index was lower in treated women (
P=0.024 for the IH group). In conclusion, the lower expression of type 2 17β-HSD mRNA in scalp hairs of untreated hirsute patients suggests androgen metabolism disturbances with predominance of more potent androgens, as occurs in men. The enzyme’s higher gene expression in treated hirsute patients could be an indirect evidence of restored enzyme activity and intracellular androgen metabolism.</description><identifier>ISSN: 0039-128X</identifier><identifier>EISSN: 1878-5867</identifier><identifier>DOI: 10.1016/S0039-128X(03)00093-X</identifier><identifier>PMID: 12957669</identifier><identifier>CODEN: STEDAM</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>17-Hydroxysteroid Dehydrogenases - biosynthesis ; 17-Hydroxysteroid Dehydrogenases - genetics ; 17β Hydroxysteroid dehydrogenase ; Adult ; Androgen receptors ; Androgens - metabolism ; Biological and medical sciences ; Case-Control Studies ; Estradiol Dehydrogenases ; Female ; Fundamental and applied biological sciences. Psychology ; Gynecology. Andrology. Obstetrics ; Hair - cytology ; Hair - enzymology ; Hair follicle ; Hirsutism ; Hirsutism - enzymology ; Hirsutism - etiology ; Hirsutism - pathology ; Humans ; Keratinocytes - enzymology ; Keratinocytes - pathology ; Male ; Medical sciences ; Polycystic Ovary Syndrome - complications ; Puberal and climacteric disorders (male and female) ; Receptors, Androgen - biosynthesis ; Receptors, Androgen - genetics ; RNA, Messenger - analysis ; Scalp - cytology ; Steroid ; Vertebrates: endocrinology</subject><ispartof>Steroids, 2003-09, Vol.68 (7), p.641-649</ispartof><rights>2003 Elsevier Inc.</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-43a5727f8d955c0ebb28bb06b9c543b5ec4840ac3742c6e3f3c461e4681235fb3</citedby><cites>FETCH-LOGICAL-c391t-43a5727f8d955c0ebb28bb06b9c543b5ec4840ac3742c6e3f3c461e4681235fb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15123210$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12957669$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oliveira, Isabel O.</creatorcontrib><creatorcontrib>Lhullier, Cintia</creatorcontrib><creatorcontrib>Brum, Ilma S.</creatorcontrib><creatorcontrib>Spritzer, Poli Mara</creatorcontrib><title>Gene expression of type 2 17β hydroxysteroid dehydrogenase in scalp hairs of hirsute women</title><title>Steroids</title><addtitle>Steroids</addtitle><description>Androgens are the main hormonal regulators of human hair growth and they are related to clinical conditions such as hirsutism. The aim of this study was to analyze the gene expression of androgen receptor (AR) and type 2 17β hydroxysteroid dehydrogenase (17β-HSD) in keratinocytes of plucked scalp hairs from hirsute patients and normal subjects. We studied 58 women with hirsutism (31 with polycystic ovary syndrome (PCOS), 27 with idiopathic hirsutism (IH)); 15 control women; and 10 control men. Hirsutism was assessed by a modified Ferriman–Gallwey method. Hormonal status was assessed between days 2 and 10 of the menstrual cycle or on any day when the patients were amenorrheic. AR and type 2 17β-HSD mRNA levels were estimated by reverse transcription-polymerase chain reaction (RT-PCR). AR expression was similar in all groups. Type 2 17β-HSD gene expression in untreated hirsute patients was lower (2.1±0.10) than in normal women (3.1±0.17), and similar to men (1.8±0.22). Comparing hirsute patients, type 2 17β-HSD expression was higher in treated PCOS (3.0±0.34 versus 2.2±0.13) and IH patients (2.5±0.19 versus 2.0±0.15); hirsutism score was lower (
P=0.003, PCOS;
P=0.003, IH); and SHBG levels were higher (
P=0.001, PCOS;
P=0.024, IH) in treated patients. The free androgen index was lower in treated women (
P=0.024 for the IH group). In conclusion, the lower expression of type 2 17β-HSD mRNA in scalp hairs of untreated hirsute patients suggests androgen metabolism disturbances with predominance of more potent androgens, as occurs in men. The enzyme’s higher gene expression in treated hirsute patients could be an indirect evidence of restored enzyme activity and intracellular androgen metabolism.</description><subject>17-Hydroxysteroid Dehydrogenases - biosynthesis</subject><subject>17-Hydroxysteroid Dehydrogenases - genetics</subject><subject>17β Hydroxysteroid dehydrogenase</subject><subject>Adult</subject><subject>Androgen receptors</subject><subject>Androgens - metabolism</subject><subject>Biological and medical sciences</subject><subject>Case-Control Studies</subject><subject>Estradiol Dehydrogenases</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Hair - cytology</subject><subject>Hair - enzymology</subject><subject>Hair follicle</subject><subject>Hirsutism</subject><subject>Hirsutism - enzymology</subject><subject>Hirsutism - etiology</subject><subject>Hirsutism - pathology</subject><subject>Humans</subject><subject>Keratinocytes - enzymology</subject><subject>Keratinocytes - pathology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Polycystic Ovary Syndrome - complications</subject><subject>Puberal and climacteric disorders (male and female)</subject><subject>Receptors, Androgen - biosynthesis</subject><subject>Receptors, Androgen - genetics</subject><subject>RNA, Messenger - analysis</subject><subject>Scalp - cytology</subject><subject>Steroid</subject><subject>Vertebrates: endocrinology</subject><issn>0039-128X</issn><issn>1878-5867</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNqFkMtKxDAUhoMoOl4eQclG0UU1lyZtViKDNxBcqDDgIqTpqROZaWrSUee1fBCfyXZm0KWrA4fvP5cPoX1KTimh8uyBEK4SyvLRMeEnhBDFk9EaGtA8yxORy2wdDX6RLbQd42sHSa7YJtqiTIlMSjVAz9dQA4bPJkCMztfYV7idN4AZptn3Fx7Py-A_57GF4F2JS1g0XqA2EbCrcbRm0uCxcSH20XFXZy3gDz-FehdtVGYSYW9Vd9DT1eXj8Ca5u7--HV7cJZYr2iYpNyJjWZWXSghLoChYXhREFsqKlBcCbJqnxFiepcxK4BW3qaSQypwyLqqC76Cj5dwm-LcZxFZPXbQwmZga_CzqjPdvK9mBYgna4GMMUOkmuKkJc02J7q3qhVXdK9OE64VVPepyB6sFs2IK5V9qpbEDDleA6YVUwdTWxT9OdJcySjrufMlBp-PdQdDROqgtlC6AbXXp3T-n_ABb0pV-</recordid><startdate>20030901</startdate><enddate>20030901</enddate><creator>Oliveira, Isabel O.</creator><creator>Lhullier, Cintia</creator><creator>Brum, Ilma S.</creator><creator>Spritzer, Poli Mara</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20030901</creationdate><title>Gene expression of type 2 17β hydroxysteroid dehydrogenase in scalp hairs of hirsute women</title><author>Oliveira, Isabel O. ; Lhullier, Cintia ; Brum, Ilma S. ; Spritzer, Poli Mara</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-43a5727f8d955c0ebb28bb06b9c543b5ec4840ac3742c6e3f3c461e4681235fb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>17-Hydroxysteroid Dehydrogenases - biosynthesis</topic><topic>17-Hydroxysteroid Dehydrogenases - genetics</topic><topic>17β Hydroxysteroid dehydrogenase</topic><topic>Adult</topic><topic>Androgen receptors</topic><topic>Androgens - metabolism</topic><topic>Biological and medical sciences</topic><topic>Case-Control Studies</topic><topic>Estradiol Dehydrogenases</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Hair - cytology</topic><topic>Hair - enzymology</topic><topic>Hair follicle</topic><topic>Hirsutism</topic><topic>Hirsutism - enzymology</topic><topic>Hirsutism - etiology</topic><topic>Hirsutism - pathology</topic><topic>Humans</topic><topic>Keratinocytes - enzymology</topic><topic>Keratinocytes - pathology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Polycystic Ovary Syndrome - complications</topic><topic>Puberal and climacteric disorders (male and female)</topic><topic>Receptors, Androgen - biosynthesis</topic><topic>Receptors, Androgen - genetics</topic><topic>RNA, Messenger - analysis</topic><topic>Scalp - cytology</topic><topic>Steroid</topic><topic>Vertebrates: endocrinology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oliveira, Isabel O.</creatorcontrib><creatorcontrib>Lhullier, Cintia</creatorcontrib><creatorcontrib>Brum, Ilma S.</creatorcontrib><creatorcontrib>Spritzer, Poli Mara</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Steroids</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oliveira, Isabel O.</au><au>Lhullier, Cintia</au><au>Brum, Ilma S.</au><au>Spritzer, Poli Mara</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gene expression of type 2 17β hydroxysteroid dehydrogenase in scalp hairs of hirsute women</atitle><jtitle>Steroids</jtitle><addtitle>Steroids</addtitle><date>2003-09-01</date><risdate>2003</risdate><volume>68</volume><issue>7</issue><spage>641</spage><epage>649</epage><pages>641-649</pages><issn>0039-128X</issn><eissn>1878-5867</eissn><coden>STEDAM</coden><abstract>Androgens are the main hormonal regulators of human hair growth and they are related to clinical conditions such as hirsutism. The aim of this study was to analyze the gene expression of androgen receptor (AR) and type 2 17β hydroxysteroid dehydrogenase (17β-HSD) in keratinocytes of plucked scalp hairs from hirsute patients and normal subjects. We studied 58 women with hirsutism (31 with polycystic ovary syndrome (PCOS), 27 with idiopathic hirsutism (IH)); 15 control women; and 10 control men. Hirsutism was assessed by a modified Ferriman–Gallwey method. Hormonal status was assessed between days 2 and 10 of the menstrual cycle or on any day when the patients were amenorrheic. AR and type 2 17β-HSD mRNA levels were estimated by reverse transcription-polymerase chain reaction (RT-PCR). AR expression was similar in all groups. Type 2 17β-HSD gene expression in untreated hirsute patients was lower (2.1±0.10) than in normal women (3.1±0.17), and similar to men (1.8±0.22). Comparing hirsute patients, type 2 17β-HSD expression was higher in treated PCOS (3.0±0.34 versus 2.2±0.13) and IH patients (2.5±0.19 versus 2.0±0.15); hirsutism score was lower (
P=0.003, PCOS;
P=0.003, IH); and SHBG levels were higher (
P=0.001, PCOS;
P=0.024, IH) in treated patients. The free androgen index was lower in treated women (
P=0.024 for the IH group). In conclusion, the lower expression of type 2 17β-HSD mRNA in scalp hairs of untreated hirsute patients suggests androgen metabolism disturbances with predominance of more potent androgens, as occurs in men. The enzyme’s higher gene expression in treated hirsute patients could be an indirect evidence of restored enzyme activity and intracellular androgen metabolism.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>12957669</pmid><doi>10.1016/S0039-128X(03)00093-X</doi><tpages>9</tpages></addata></record> |
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subjects | 17-Hydroxysteroid Dehydrogenases - biosynthesis 17-Hydroxysteroid Dehydrogenases - genetics 17β Hydroxysteroid dehydrogenase Adult Androgen receptors Androgens - metabolism Biological and medical sciences Case-Control Studies Estradiol Dehydrogenases Female Fundamental and applied biological sciences. Psychology Gynecology. Andrology. Obstetrics Hair - cytology Hair - enzymology Hair follicle Hirsutism Hirsutism - enzymology Hirsutism - etiology Hirsutism - pathology Humans Keratinocytes - enzymology Keratinocytes - pathology Male Medical sciences Polycystic Ovary Syndrome - complications Puberal and climacteric disorders (male and female) Receptors, Androgen - biosynthesis Receptors, Androgen - genetics RNA, Messenger - analysis Scalp - cytology Steroid Vertebrates: endocrinology |
title | Gene expression of type 2 17β hydroxysteroid dehydrogenase in scalp hairs of hirsute women |
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