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Gene expression of type 2 17β hydroxysteroid dehydrogenase in scalp hairs of hirsute women

Androgens are the main hormonal regulators of human hair growth and they are related to clinical conditions such as hirsutism. The aim of this study was to analyze the gene expression of androgen receptor (AR) and type 2 17β hydroxysteroid dehydrogenase (17β-HSD) in keratinocytes of plucked scalp ha...

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Published in:Steroids 2003-09, Vol.68 (7), p.641-649
Main Authors: Oliveira, Isabel O., Lhullier, Cintia, Brum, Ilma S., Spritzer, Poli Mara
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creator Oliveira, Isabel O.
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description Androgens are the main hormonal regulators of human hair growth and they are related to clinical conditions such as hirsutism. The aim of this study was to analyze the gene expression of androgen receptor (AR) and type 2 17β hydroxysteroid dehydrogenase (17β-HSD) in keratinocytes of plucked scalp hairs from hirsute patients and normal subjects. We studied 58 women with hirsutism (31 with polycystic ovary syndrome (PCOS), 27 with idiopathic hirsutism (IH)); 15 control women; and 10 control men. Hirsutism was assessed by a modified Ferriman–Gallwey method. Hormonal status was assessed between days 2 and 10 of the menstrual cycle or on any day when the patients were amenorrheic. AR and type 2 17β-HSD mRNA levels were estimated by reverse transcription-polymerase chain reaction (RT-PCR). AR expression was similar in all groups. Type 2 17β-HSD gene expression in untreated hirsute patients was lower (2.1±0.10) than in normal women (3.1±0.17), and similar to men (1.8±0.22). Comparing hirsute patients, type 2 17β-HSD expression was higher in treated PCOS (3.0±0.34 versus 2.2±0.13) and IH patients (2.5±0.19 versus 2.0±0.15); hirsutism score was lower ( P=0.003, PCOS; P=0.003, IH); and SHBG levels were higher ( P=0.001, PCOS; P=0.024, IH) in treated patients. The free androgen index was lower in treated women ( P=0.024 for the IH group). In conclusion, the lower expression of type 2 17β-HSD mRNA in scalp hairs of untreated hirsute patients suggests androgen metabolism disturbances with predominance of more potent androgens, as occurs in men. The enzyme’s higher gene expression in treated hirsute patients could be an indirect evidence of restored enzyme activity and intracellular androgen metabolism.
doi_str_mv 10.1016/S0039-128X(03)00093-X
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The aim of this study was to analyze the gene expression of androgen receptor (AR) and type 2 17β hydroxysteroid dehydrogenase (17β-HSD) in keratinocytes of plucked scalp hairs from hirsute patients and normal subjects. We studied 58 women with hirsutism (31 with polycystic ovary syndrome (PCOS), 27 with idiopathic hirsutism (IH)); 15 control women; and 10 control men. Hirsutism was assessed by a modified Ferriman–Gallwey method. Hormonal status was assessed between days 2 and 10 of the menstrual cycle or on any day when the patients were amenorrheic. AR and type 2 17β-HSD mRNA levels were estimated by reverse transcription-polymerase chain reaction (RT-PCR). AR expression was similar in all groups. Type 2 17β-HSD gene expression in untreated hirsute patients was lower (2.1±0.10) than in normal women (3.1±0.17), and similar to men (1.8±0.22). Comparing hirsute patients, type 2 17β-HSD expression was higher in treated PCOS (3.0±0.34 versus 2.2±0.13) and IH patients (2.5±0.19 versus 2.0±0.15); hirsutism score was lower ( P=0.003, PCOS; P=0.003, IH); and SHBG levels were higher ( P=0.001, PCOS; P=0.024, IH) in treated patients. The free androgen index was lower in treated women ( P=0.024 for the IH group). In conclusion, the lower expression of type 2 17β-HSD mRNA in scalp hairs of untreated hirsute patients suggests androgen metabolism disturbances with predominance of more potent androgens, as occurs in men. 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The aim of this study was to analyze the gene expression of androgen receptor (AR) and type 2 17β hydroxysteroid dehydrogenase (17β-HSD) in keratinocytes of plucked scalp hairs from hirsute patients and normal subjects. We studied 58 women with hirsutism (31 with polycystic ovary syndrome (PCOS), 27 with idiopathic hirsutism (IH)); 15 control women; and 10 control men. Hirsutism was assessed by a modified Ferriman–Gallwey method. Hormonal status was assessed between days 2 and 10 of the menstrual cycle or on any day when the patients were amenorrheic. AR and type 2 17β-HSD mRNA levels were estimated by reverse transcription-polymerase chain reaction (RT-PCR). AR expression was similar in all groups. Type 2 17β-HSD gene expression in untreated hirsute patients was lower (2.1±0.10) than in normal women (3.1±0.17), and similar to men (1.8±0.22). Comparing hirsute patients, type 2 17β-HSD expression was higher in treated PCOS (3.0±0.34 versus 2.2±0.13) and IH patients (2.5±0.19 versus 2.0±0.15); hirsutism score was lower ( P=0.003, PCOS; P=0.003, IH); and SHBG levels were higher ( P=0.001, PCOS; P=0.024, IH) in treated patients. The free androgen index was lower in treated women ( P=0.024 for the IH group). In conclusion, the lower expression of type 2 17β-HSD mRNA in scalp hairs of untreated hirsute patients suggests androgen metabolism disturbances with predominance of more potent androgens, as occurs in men. 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Obstetrics</topic><topic>Hair - cytology</topic><topic>Hair - enzymology</topic><topic>Hair follicle</topic><topic>Hirsutism</topic><topic>Hirsutism - enzymology</topic><topic>Hirsutism - etiology</topic><topic>Hirsutism - pathology</topic><topic>Humans</topic><topic>Keratinocytes - enzymology</topic><topic>Keratinocytes - pathology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Polycystic Ovary Syndrome - complications</topic><topic>Puberal and climacteric disorders (male and female)</topic><topic>Receptors, Androgen - biosynthesis</topic><topic>Receptors, Androgen - genetics</topic><topic>RNA, Messenger - analysis</topic><topic>Scalp - cytology</topic><topic>Steroid</topic><topic>Vertebrates: endocrinology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oliveira, Isabel O.</creatorcontrib><creatorcontrib>Lhullier, Cintia</creatorcontrib><creatorcontrib>Brum, Ilma S.</creatorcontrib><creatorcontrib>Spritzer, Poli Mara</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Steroids</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oliveira, Isabel O.</au><au>Lhullier, Cintia</au><au>Brum, Ilma S.</au><au>Spritzer, Poli Mara</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gene expression of type 2 17β hydroxysteroid dehydrogenase in scalp hairs of hirsute women</atitle><jtitle>Steroids</jtitle><addtitle>Steroids</addtitle><date>2003-09-01</date><risdate>2003</risdate><volume>68</volume><issue>7</issue><spage>641</spage><epage>649</epage><pages>641-649</pages><issn>0039-128X</issn><eissn>1878-5867</eissn><coden>STEDAM</coden><abstract>Androgens are the main hormonal regulators of human hair growth and they are related to clinical conditions such as hirsutism. The aim of this study was to analyze the gene expression of androgen receptor (AR) and type 2 17β hydroxysteroid dehydrogenase (17β-HSD) in keratinocytes of plucked scalp hairs from hirsute patients and normal subjects. We studied 58 women with hirsutism (31 with polycystic ovary syndrome (PCOS), 27 with idiopathic hirsutism (IH)); 15 control women; and 10 control men. Hirsutism was assessed by a modified Ferriman–Gallwey method. Hormonal status was assessed between days 2 and 10 of the menstrual cycle or on any day when the patients were amenorrheic. AR and type 2 17β-HSD mRNA levels were estimated by reverse transcription-polymerase chain reaction (RT-PCR). AR expression was similar in all groups. Type 2 17β-HSD gene expression in untreated hirsute patients was lower (2.1±0.10) than in normal women (3.1±0.17), and similar to men (1.8±0.22). Comparing hirsute patients, type 2 17β-HSD expression was higher in treated PCOS (3.0±0.34 versus 2.2±0.13) and IH patients (2.5±0.19 versus 2.0±0.15); hirsutism score was lower ( P=0.003, PCOS; P=0.003, IH); and SHBG levels were higher ( P=0.001, PCOS; P=0.024, IH) in treated patients. The free androgen index was lower in treated women ( P=0.024 for the IH group). In conclusion, the lower expression of type 2 17β-HSD mRNA in scalp hairs of untreated hirsute patients suggests androgen metabolism disturbances with predominance of more potent androgens, as occurs in men. The enzyme’s higher gene expression in treated hirsute patients could be an indirect evidence of restored enzyme activity and intracellular androgen metabolism.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>12957669</pmid><doi>10.1016/S0039-128X(03)00093-X</doi><tpages>9</tpages></addata></record>
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subjects 17-Hydroxysteroid Dehydrogenases - biosynthesis
17-Hydroxysteroid Dehydrogenases - genetics
17β Hydroxysteroid dehydrogenase
Adult
Androgen receptors
Androgens - metabolism
Biological and medical sciences
Case-Control Studies
Estradiol Dehydrogenases
Female
Fundamental and applied biological sciences. Psychology
Gynecology. Andrology. Obstetrics
Hair - cytology
Hair - enzymology
Hair follicle
Hirsutism
Hirsutism - enzymology
Hirsutism - etiology
Hirsutism - pathology
Humans
Keratinocytes - enzymology
Keratinocytes - pathology
Male
Medical sciences
Polycystic Ovary Syndrome - complications
Puberal and climacteric disorders (male and female)
Receptors, Androgen - biosynthesis
Receptors, Androgen - genetics
RNA, Messenger - analysis
Scalp - cytology
Steroid
Vertebrates: endocrinology
title Gene expression of type 2 17β hydroxysteroid dehydrogenase in scalp hairs of hirsute women
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