lipopolysaccharide from Aspergillus flavus conidia

A lipopolysaccharide was isolated by extraction of Aspergillus flavus conidia with 45% phenol at 68-70 degrees C. Quantitative analysis revealed 7% nucleic acids, 5.5% proteins, 46% polysaccharides and 49% liquids, of which 12% were covalently bound. Glucose, mannose, galactose and fucose were detec...

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Bibliographic Details
Published in:Folia microbiologica 1981-01, Vol.26 (3), p.212-216
Main Authors: Budinska, O, Kubin, V, Franek, J, Julak, J, Panos, J
Format: Article
Language:English
Subjects:
Aspergillus flavus - metabolism
Escherichia coli - metabolism
Fatty Acids - analysis
fungi
Lipids - analysis
Lipopolysaccharides - analysis
Monosaccharides - analysis
Polysaccharides, Bacterial - analysis
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Summary:A lipopolysaccharide was isolated by extraction of Aspergillus flavus conidia with 45% phenol at 68-70 degrees C. Quantitative analysis revealed 7% nucleic acids, 5.5% proteins, 46% polysaccharides and 49% liquids, of which 12% were covalently bound. Glucose, mannose, galactose and fucose were detected as monosaccharide components of the polysaccharide moiety by gas chromatography; palmitic acid, stearic acid, oleic acid, linoleic acid and myristic acid were mainly present in the lipidic fraction. This material differs from the bacterial lipopolysaccharides, both in composition of the polysaccharide moiety and representation of fatty acids in the lipidic fraction.
ISSN:0015-5632
1874-9356
DOI:10.1007/BF02927426