Rice gene expression in response to N-acetylchitooligosaccharide elicitor: comprehensive analysis by DNA microarray with randomly selected ESTs

• Published in: Plant molecular biology 2003-06, Vol.52 (3), p.537-551
• Main Authors: Akimoto-Tomiyama, Chiharu, Sakata, Katsumi, Yazaki, Junshi, Nakamura, Keiko, Fujii, Fumiko, Shimbo, Kanako, Yamamoto, Kimiko, Sasaki, Takuji, Kishimoto, Naoki, Kikuchi, Shoshi, Shibuya, Naoto, Minami, Eiichi
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Blotting, Northern; Cells, Cultured; Expressed Sequence Tags; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Plant - drug effects; Genes, Plant - genetics; Genomics; Molecular Sequence Data; Oligonucleotide Array Sequence Analysis - methods; Oligosaccharides - pharmacology; Oryza - cytology; Oryza - genetics; Protein Kinases - genetics; RNA, Plant - genetics; RNA, Plant - metabolism; Sequence Homology, Amino Acid
• ISSN: 0167-4412; 1573-5028
• DOI: 10.1023/A:1024890601888

N-acetylchitooligosaccharides are potent elicitors to suspension-cultured rice cells, inducing a set of defense reactions. Expression of defense-related genes is considered to play an important role in defense reactions, and we employed microarray analysis of 8987 randomly selected expressed sequence tags to analyze the changes in gene expression caused by N-acetylchitooctaose. In this experiment, 166 genes were significantly induced and 93 genes were repressed. RNA gel blot analysis of 16 of these genes confirmed the microarray results. Of the 259 ESTs identified as responsive to N-acetylchytooctaose, 18 genes are related to signal transduction, including five calcium-dependent protein kinases (CDPKs). Among these, three novel CDPKs responsive to N-acetylchitooctaose were isolated.