Developmentally induced changes of the proteome in the protozoan parasite Leishmania donovani

• Published in: Proteomics (Weinheim) 2003-09, Vol.3 (9), p.1811-1829
• Main Authors: Bente, Meike, Harder, Simone, Wiesgigl, Martina, Heukeshoven, Jochen, Gelhaus, Christoph, Krause, Eberhard, Clos, Joachim, Bruchhaus, Iris
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Cell Cycle Proteins - analysis; Cell Differentiation; Cell Membrane - chemistry; Cytoskeleton - chemistry; Electrophoresis, Gel, Two-Dimensional; Energy Metabolism; Fundamental and applied biological sciences. Psychology; Heat shock protein 90; HSP90 Heat-Shock Proteins - antagonists & inhibitors; Humans; Leishmania donovani; Leishmania donovani - chemistry; Leishmania donovani - growth & development; Leishmania donovani - metabolism; Life cycle. Host-agent relationship. Pathogenesis; Proteome; Proteome - analysis; Proteome - metabolism; Protozoa; Protozoan Proteins - analysis; Protozoan Proteins - metabolism; Stage differentiation; Stage-specific proteins
• ISSN: 1615-9853; 1615-9861
• DOI: 10.1002/pmic.200300462

In order to proceed through their life cycle, protozoan parasites of the genus Leishmania cycle between sandflies and mammals. This change of environment correlates with the differentiation from the promastigote stage (insect form) to the amastigote stage (intracellular mammalian form). The molecular basis underlying this major transformation is poorly understood so far; however, heat shock protein 90 (HSP90) appears to play a pivotal role. To further elucidate this process we identified proteins expressed preferentially in either of the two life cycle stages. By using two‐dimensional (2‐D) gel electrophoresis we observed defined changes in the protein pattern. A total of approximately 2000 protein spots were visualized. Of these, 31 proteins were present only in promastigotes. The abundance of 65 proteins increased during heat‐induced in vitro amastigote differentiation, while a decreased abundance is observed for four proteins late in amastigote differentiation. Further analyses using matrix‐assisted laser desorption/ionization‐time of flight mass spectrometry and peptide mass fingerprinting 67 protein spots were identified representing 41 different proteins known from databases and eight hypothetical proteins. Further studies showed that most of the stage‐specific proteins fall into five groups of functionally related proteins. These functional categories are: (i) stress response (e.g. heat, oxidative stress); (ii) cytoskeleton and cell membrane; (iii) energy metabolism and phosphorylation; (iv) cell cycle and proliferation; and (v) amino acid metabolism. Very similar changes in the 2‐D protein pattern were obtained when in vitro amastigote differentiation was induced either by pharmacological inhibition of HSP90 or by a combination of heat stress and acidic pH supporting the critical role for HSP90 in life cycle control.