Capillary high-performance liquid chromatographic determination of lutein and zeaxanthin in aqueous humor from a single mouse eye

To protect the eye from ultraviolet phototoxicity caused by free radicals, ocular components such as the aqueous humor accumulate antioxidants, such as the carotenoids. Lutein and zeaxanthin are the only carotenoids known to be present in the aqueous humor. Due to the small sample volume, pooling of...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2003-09, Vol.795 (1), p.17-23
Main Authors: Karlsen, Anette, Alexander, George, Blomhoff, Rune, Gundersen, Thomas E
Format: Article
Language:English
Subjects:
Animals
Aqueous Humor - chemistry
beta Carotene - analogs & derivatives
beta Carotene - analysis
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
Environmental pollutants toxicology
General aspects
Lutein
Lutein - analysis
Medical sciences
Mice
Reference Standards
Reproducibility of Results
Sensitivity and Specificity
Toxicology
Xanthophylls
Zeaxanthin
Zeaxanthins
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Summary:To protect the eye from ultraviolet phototoxicity caused by free radicals, ocular components such as the aqueous humor accumulate antioxidants, such as the carotenoids. Lutein and zeaxanthin are the only carotenoids known to be present in the aqueous humor. Due to the small sample volume, pooling of samples from an undesirable large number of animals is often required for sufficient sensitivity and statistically significant differences to be achieved. In this paper we present a rapid, sensitive and robust packed capillary high-performance liquid chromatographic visible detection method for the quantification of lutein and zeaxanthin in the aqueous humor of single mouse eyes.
ISSN:1570-0232
1873-376X
DOI:10.1016/S1570-0232(03)00483-5