mammalian-like lipase gene is expressed in the female reproductive accessory glands of the sand fly Phlebotomus papatasi (Diptera, Psychodidae)

A lipase-like protein (PhpaLIP) was identified as the major protein component in the secretion of the female reproductive accessory glands of the sand fly Phlebotomus papatasi. The full-length cDNA encoding this protein was isolated and its nucleotide sequence determined. The deduced translational p...

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Bibliographic Details
Published in:Insect molecular biology 2003-10, Vol.12 (5), p.501-508
Main Authors: Rosetto, M, Belardinelli, M, Fausto, A.M, Marchini, D, Bongiorno, G, Maroli, M, Mazzini, M
Format: Article
Language:English
Subjects:
accessory glands
accessory sex glands
amino acid sequences
Animals
Base Sequence
carboxylic ester hydrolases
complementary DNA
DNA Primers
DNA, Complementary - genetics
Electrophoresis, Polyacrylamide Gel
enzyme activity
Female
female reproductive system
gene expression
Genitalia, Female - secretion
insect vectors
lipase
Lipase - genetics
messenger RNA
Molecular Sequence Data
nucleotide sequences
Phlebotomus - enzymology
Phlebotomus - genetics
Phlebotomus papatasi
protein secretion
reproduction
Reverse Transcriptase Polymerase Chain Reaction
sand fly
Sequence Alignment
Sequence Analysis, DNA
sequence homology
Spectrophotometry
structural genes
Transcription, Genetic - genetics
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Summary:A lipase-like protein (PhpaLIP) was identified as the major protein component in the secretion of the female reproductive accessory glands of the sand fly Phlebotomus papatasi. The full-length cDNA encoding this protein was isolated and its nucleotide sequence determined. The deduced translational product of the gene contains a GFSFG motif, consistent with a GXSXG consensus, which is shared by most bacterial and eukaryotic hydrolases. Transcriptional analysis of the PhpaLIP gene showed that its expression is female-specific, and is also detectable in districts other than accessory glands, suggesting that it might play different functions. Taken together with the observation of sequence similarity shared by PhpaLIP and mammalian lipases, the demonstration of the presence of lipase activity in the accessory gland secretion suggests a possible biological role of PhpaLIP gene product.
ISSN:0962-1075
1365-2583
DOI:10.1046/j.1365-2583.2003.00436.x