Two-dimensional nano-liquid chromatography–mass spectrometry system for applications in proteomics

This work demonstrates the development of a method for the analysis of complex proteome samples by two-dimensional nano-liquid chromatography–mass spectrometry. This approach includes strong cation-exchange, sample enrichment, reversed-phase chromatography and nanospray ion trap mass spectroscopy wi...

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Bibliographic Details
Published in:Journal of Chromatography A 2003-08, Vol.1009 (1), p.197-205
Main Authors: Nägele, Edgar, Vollmer, Martin, Hörth, Patric
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Cattle
Chromatography, High Pressure Liquid - methods
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Mass Spectrometry - methods
Molecular Sequence Data
Nanotechnology
Proteins
Proteins - chemistry
Proteins - isolation & purification
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Summary:This work demonstrates the development of a method for the analysis of complex proteome samples by two-dimensional nano-liquid chromatography–mass spectrometry. This approach includes strong cation-exchange, sample enrichment, reversed-phase chromatography and nanospray ion trap mass spectroscopy with data dependent tandem mass spectrometry spectra acquisition, and subsequent database search. The new methodology was first evaluated using standard protein digest samples. Finally, data for the analysis of a total Escherichia coli proteome are provided.
ISSN:0021-9673
DOI:10.1016/S0021-9673(03)01034-3