Loading…

Campylobacter-stimulated INT407 cells produce dissociated cytokine profiles

Objectives. To study the action of factors produced by living Campylobacter jejuni ( C. jejuni) against those present within sonicated and filtrated bacteria on induction of potential cytokines by the human intestinal cell line INT407. Methods. We used immunohistochemical technique modified to detec...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of infection 2003-10, Vol.47 (3), p.217-224
Main Authors: Al-Salloom, Fajer Subah, Al Mahmeed, Ali, Ismaeel, Abdulrahman, Botta, Giuseppe A, Bakhiet, Moiz
Format: Article
Language:English
Subjects:
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c540t-e405bd6bddcc0d116445e84c0902e4521445aa752f611f2eb623e30f2c3a1eab3
cites
container_end_page 224
container_issue 3
container_start_page 217
container_title The Journal of infection
container_volume 47
creator Al-Salloom, Fajer Subah
Al Mahmeed, Ali
Ismaeel, Abdulrahman
Botta, Giuseppe A
Bakhiet, Moiz
description Objectives. To study the action of factors produced by living Campylobacter jejuni ( C. jejuni) against those present within sonicated and filtrated bacteria on induction of potential cytokines by the human intestinal cell line INT407. Methods. We used immunohistochemical technique modified to detect intracellular production of cytokines protein and RT-PCR to read RNA messages for evaluation of de novo cytokine synthesis. Results. The data herein display dissociation of cytokine profiles induced on by living C. jejuni. Exposure of INT407 cells to 10 6 live bacteria showed the highest numbers of cytokine producing cells of all examined cytokines. IFN-γ was the highest induced cytokine followed by IL-10, TNF-α and lastly IL-4. Also, abrogation of induction of the proinflammatory cytokines IFN-γ and TNF-α but not the antiinflammatory cytokines IL-4 and IL-10 by sonicated and filtrated bacteria was depicted. At the mRNA level, TNF-α signals were noted in accordance with its protein levels since increased TNF-α mRNA signals were registered only after stimulation with living bacteria. Very low or no induction of TNF-α was registered with non-stimulated cells. Conclusions. These results illustrate for the first time a role for factors from living bacteria in directing the immune response towards Th1 type. Characterization of such factors may be essential for future immunotherapeutic interventions during severe bacterial infections.
doi_str_mv 10.1016/S0163-4453(03)00076-8
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73647489</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0163445303000768</els_id><sourcerecordid>73647489</sourcerecordid><originalsourceid>FETCH-LOGICAL-c540t-e405bd6bddcc0d116445e84c0902e4521445aa752f611f2eb623e30f2c3a1eab3</originalsourceid><addsrcrecordid>eNqFkF1LwzAUhoMobk5_grIbRS-q-W57JTL8GA69cF6HNDmFaLvOpBX270234S4HIeGQ55zz8iB0TvAtwUTefcSLJZwLdo3ZDcY4lUl2gIZEMJrQlNNDNPxHBugkhK8I5SyXx2hAaC4Zy9gQvU50vVxVTaFNCz4Jrau7Srdgx9O3Ocfp2EBVhfHSN7YzMLYuhMa4NWBWbfPtFtB_lq6CcIqOSl0FONu-I_T59DifvCSz9-fp5GGWGMFxmwDHorCysNYYbAmRMSFk3OAcU-CCklhrnQpaSkJKCoWkDBguqWGagC7YCF1t5sbFPx2EVtUu9Dn1ApouqJRJnvIs3wuSLEsF4zyCYgMa34TgoVRL72rtV4pg1etWa92qd6lwPL1ulcW-i-2CrqjB7rq2fiNwuQV0MLoqvV4YF3acIJTkWT_ofsNB9PbrwKtgHCwMWOfBtMo2bk-UP8Ftm7c</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>18875344</pqid></control><display><type>article</type><title>Campylobacter-stimulated INT407 cells produce dissociated cytokine profiles</title><source>ScienceDirect Journals</source><creator>Al-Salloom, Fajer Subah ; Al Mahmeed, Ali ; Ismaeel, Abdulrahman ; Botta, Giuseppe A ; Bakhiet, Moiz</creator><creatorcontrib>Al-Salloom, Fajer Subah ; Al Mahmeed, Ali ; Ismaeel, Abdulrahman ; Botta, Giuseppe A ; Bakhiet, Moiz</creatorcontrib><description>Objectives. To study the action of factors produced by living Campylobacter jejuni ( C. jejuni) against those present within sonicated and filtrated bacteria on induction of potential cytokines by the human intestinal cell line INT407. Methods. We used immunohistochemical technique modified to detect intracellular production of cytokines protein and RT-PCR to read RNA messages for evaluation of de novo cytokine synthesis. Results. The data herein display dissociation of cytokine profiles induced on by living C. jejuni. Exposure of INT407 cells to 10 6 live bacteria showed the highest numbers of cytokine producing cells of all examined cytokines. IFN-γ was the highest induced cytokine followed by IL-10, TNF-α and lastly IL-4. Also, abrogation of induction of the proinflammatory cytokines IFN-γ and TNF-α but not the antiinflammatory cytokines IL-4 and IL-10 by sonicated and filtrated bacteria was depicted. At the mRNA level, TNF-α signals were noted in accordance with its protein levels since increased TNF-α mRNA signals were registered only after stimulation with living bacteria. Very low or no induction of TNF-α was registered with non-stimulated cells. Conclusions. These results illustrate for the first time a role for factors from living bacteria in directing the immune response towards Th1 type. Characterization of such factors may be essential for future immunotherapeutic interventions during severe bacterial infections.</description><identifier>ISSN: 0163-4453</identifier><identifier>EISSN: 1532-2742</identifier><identifier>DOI: 10.1016/S0163-4453(03)00076-8</identifier><identifier>PMID: 12963383</identifier><identifier>CODEN: JINFD2</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Bacteriology ; Biogenesis of cell structures, supramolecular organization ; Biological and medical sciences ; Campylobacter jejuni ; Campylobacter jejuni - immunology ; Campylobacter jejuni - physiology ; Cells, Cultured ; Diarrhea ; Fundamental and applied biological sciences. Psychology ; Humans ; IFN-γ ; IL-10 ; IL-4 ; Interferon-gamma - biosynthesis ; Intestinal Mucosa - metabolism ; Intestines - immunology ; Intestines - microbiology ; Microbiology ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - biosynthesis ; TNF-α ; Tumor Necrosis Factor-alpha - biosynthesis</subject><ispartof>The Journal of infection, 2003-10, Vol.47 (3), p.217-224</ispartof><rights>2003 The British Infection Society</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c540t-e405bd6bddcc0d116445e84c0902e4521445aa752f611f2eb623e30f2c3a1eab3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=15121988$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12963383$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Al-Salloom, Fajer Subah</creatorcontrib><creatorcontrib>Al Mahmeed, Ali</creatorcontrib><creatorcontrib>Ismaeel, Abdulrahman</creatorcontrib><creatorcontrib>Botta, Giuseppe A</creatorcontrib><creatorcontrib>Bakhiet, Moiz</creatorcontrib><title>Campylobacter-stimulated INT407 cells produce dissociated cytokine profiles</title><title>The Journal of infection</title><addtitle>J Infect</addtitle><description>Objectives. To study the action of factors produced by living Campylobacter jejuni ( C. jejuni) against those present within sonicated and filtrated bacteria on induction of potential cytokines by the human intestinal cell line INT407. Methods. We used immunohistochemical technique modified to detect intracellular production of cytokines protein and RT-PCR to read RNA messages for evaluation of de novo cytokine synthesis. Results. The data herein display dissociation of cytokine profiles induced on by living C. jejuni. Exposure of INT407 cells to 10 6 live bacteria showed the highest numbers of cytokine producing cells of all examined cytokines. IFN-γ was the highest induced cytokine followed by IL-10, TNF-α and lastly IL-4. Also, abrogation of induction of the proinflammatory cytokines IFN-γ and TNF-α but not the antiinflammatory cytokines IL-4 and IL-10 by sonicated and filtrated bacteria was depicted. At the mRNA level, TNF-α signals were noted in accordance with its protein levels since increased TNF-α mRNA signals were registered only after stimulation with living bacteria. Very low or no induction of TNF-α was registered with non-stimulated cells. Conclusions. These results illustrate for the first time a role for factors from living bacteria in directing the immune response towards Th1 type. Characterization of such factors may be essential for future immunotherapeutic interventions during severe bacterial infections.</description><subject>Bacteriology</subject><subject>Biogenesis of cell structures, supramolecular organization</subject><subject>Biological and medical sciences</subject><subject>Campylobacter jejuni</subject><subject>Campylobacter jejuni - immunology</subject><subject>Campylobacter jejuni - physiology</subject><subject>Cells, Cultured</subject><subject>Diarrhea</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>IFN-γ</subject><subject>IL-10</subject><subject>IL-4</subject><subject>Interferon-gamma - biosynthesis</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Intestines - immunology</subject><subject>Intestines - microbiology</subject><subject>Microbiology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - biosynthesis</subject><subject>TNF-α</subject><subject>Tumor Necrosis Factor-alpha - biosynthesis</subject><issn>0163-4453</issn><issn>1532-2742</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNqFkF1LwzAUhoMobk5_grIbRS-q-W57JTL8GA69cF6HNDmFaLvOpBX270234S4HIeGQ55zz8iB0TvAtwUTefcSLJZwLdo3ZDcY4lUl2gIZEMJrQlNNDNPxHBugkhK8I5SyXx2hAaC4Zy9gQvU50vVxVTaFNCz4Jrau7Srdgx9O3Ocfp2EBVhfHSN7YzMLYuhMa4NWBWbfPtFtB_lq6CcIqOSl0FONu-I_T59DifvCSz9-fp5GGWGMFxmwDHorCysNYYbAmRMSFk3OAcU-CCklhrnQpaSkJKCoWkDBguqWGagC7YCF1t5sbFPx2EVtUu9Dn1ApouqJRJnvIs3wuSLEsF4zyCYgMa34TgoVRL72rtV4pg1etWa92qd6lwPL1ulcW-i-2CrqjB7rq2fiNwuQV0MLoqvV4YF3acIJTkWT_ofsNB9PbrwKtgHCwMWOfBtMo2bk-UP8Ftm7c</recordid><startdate>20031001</startdate><enddate>20031001</enddate><creator>Al-Salloom, Fajer Subah</creator><creator>Al Mahmeed, Ali</creator><creator>Ismaeel, Abdulrahman</creator><creator>Botta, Giuseppe A</creator><creator>Bakhiet, Moiz</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20031001</creationdate><title>Campylobacter-stimulated INT407 cells produce dissociated cytokine profiles</title><author>Al-Salloom, Fajer Subah ; Al Mahmeed, Ali ; Ismaeel, Abdulrahman ; Botta, Giuseppe A ; Bakhiet, Moiz</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c540t-e405bd6bddcc0d116445e84c0902e4521445aa752f611f2eb623e30f2c3a1eab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Bacteriology</topic><topic>Biogenesis of cell structures, supramolecular organization</topic><topic>Biological and medical sciences</topic><topic>Campylobacter jejuni</topic><topic>Campylobacter jejuni - immunology</topic><topic>Campylobacter jejuni - physiology</topic><topic>Cells, Cultured</topic><topic>Diarrhea</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>IFN-γ</topic><topic>IL-10</topic><topic>IL-4</topic><topic>Interferon-gamma - biosynthesis</topic><topic>Intestinal Mucosa - metabolism</topic><topic>Intestines - immunology</topic><topic>Intestines - microbiology</topic><topic>Microbiology</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - biosynthesis</topic><topic>TNF-α</topic><topic>Tumor Necrosis Factor-alpha - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Al-Salloom, Fajer Subah</creatorcontrib><creatorcontrib>Al Mahmeed, Ali</creatorcontrib><creatorcontrib>Ismaeel, Abdulrahman</creatorcontrib><creatorcontrib>Botta, Giuseppe A</creatorcontrib><creatorcontrib>Bakhiet, Moiz</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Al-Salloom, Fajer Subah</au><au>Al Mahmeed, Ali</au><au>Ismaeel, Abdulrahman</au><au>Botta, Giuseppe A</au><au>Bakhiet, Moiz</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Campylobacter-stimulated INT407 cells produce dissociated cytokine profiles</atitle><jtitle>The Journal of infection</jtitle><addtitle>J Infect</addtitle><date>2003-10-01</date><risdate>2003</risdate><volume>47</volume><issue>3</issue><spage>217</spage><epage>224</epage><pages>217-224</pages><issn>0163-4453</issn><eissn>1532-2742</eissn><coden>JINFD2</coden><abstract>Objectives. To study the action of factors produced by living Campylobacter jejuni ( C. jejuni) against those present within sonicated and filtrated bacteria on induction of potential cytokines by the human intestinal cell line INT407. Methods. We used immunohistochemical technique modified to detect intracellular production of cytokines protein and RT-PCR to read RNA messages for evaluation of de novo cytokine synthesis. Results. The data herein display dissociation of cytokine profiles induced on by living C. jejuni. Exposure of INT407 cells to 10 6 live bacteria showed the highest numbers of cytokine producing cells of all examined cytokines. IFN-γ was the highest induced cytokine followed by IL-10, TNF-α and lastly IL-4. Also, abrogation of induction of the proinflammatory cytokines IFN-γ and TNF-α but not the antiinflammatory cytokines IL-4 and IL-10 by sonicated and filtrated bacteria was depicted. At the mRNA level, TNF-α signals were noted in accordance with its protein levels since increased TNF-α mRNA signals were registered only after stimulation with living bacteria. Very low or no induction of TNF-α was registered with non-stimulated cells. Conclusions. These results illustrate for the first time a role for factors from living bacteria in directing the immune response towards Th1 type. Characterization of such factors may be essential for future immunotherapeutic interventions during severe bacterial infections.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>12963383</pmid><doi>10.1016/S0163-4453(03)00076-8</doi><tpages>8</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0163-4453
ispartof The Journal of infection, 2003-10, Vol.47 (3), p.217-224
issn 0163-4453
1532-2742
language eng
recordid cdi_proquest_miscellaneous_73647489
source ScienceDirect Journals
subjects Bacteriology
Biogenesis of cell structures, supramolecular organization
Biological and medical sciences
Campylobacter jejuni
Campylobacter jejuni - immunology
Campylobacter jejuni - physiology
Cells, Cultured
Diarrhea
Fundamental and applied biological sciences. Psychology
Humans
IFN-γ
IL-10
IL-4
Interferon-gamma - biosynthesis
Intestinal Mucosa - metabolism
Intestines - immunology
Intestines - microbiology
Microbiology
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - biosynthesis
TNF-α
Tumor Necrosis Factor-alpha - biosynthesis
title Campylobacter-stimulated INT407 cells produce dissociated cytokine profiles
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T21%3A10%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Campylobacter-stimulated%20INT407%20cells%20produce%20dissociated%20cytokine%20profiles&rft.jtitle=The%20Journal%20of%20infection&rft.au=Al-Salloom,%20Fajer%20Subah&rft.date=2003-10-01&rft.volume=47&rft.issue=3&rft.spage=217&rft.epage=224&rft.pages=217-224&rft.issn=0163-4453&rft.eissn=1532-2742&rft.coden=JINFD2&rft_id=info:doi/10.1016/S0163-4453(03)00076-8&rft_dat=%3Cproquest_cross%3E73647489%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c540t-e405bd6bddcc0d116445e84c0902e4521445aa752f611f2eb623e30f2c3a1eab3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=18875344&rft_id=info:pmid/12963383&rfr_iscdi=true