Phylogenetic relationships among 28 spirotrichous ciliates documented by rDNA

The contiguous sequence of the SSU rDNA, ITS 1, 5.8S, ITS 2, and ∼1370 bp at the 5 ′ end of the LSU rDNA was determined in 25 stichotrichs, one oligotrich, and two hypotrichs. Maximum parsimony, neighbor-joining, and quartet-puzzling analyses were used to construct individual phylogenetic trees for...

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Bibliographic Details
Published in:Molecular phylogenetics and evolution 2003-11, Vol.29 (2), p.258-267
Main Authors: Hewitt, Elizabeth A., Müller, Kirsten M., Cannone, Jamie, Hogan, Daniel J., Gutell, Robin, Prescott, David M.
Format: Article
Language:English
Subjects:
Animals
Cell Nucleus - metabolism
Ciliophora - genetics
Ciliophora - physiology
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
DNA, Ribosomal Spacer
Halteria grandinella
Holosticha polystylata
Oxytricha granulifera
Oxytricha longa
Phylogeny
rRNA
Species Specificity
Stichotrichida
Uroleptus gallina
Uroleptus pisces
Urostyla grandis
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Summary:The contiguous sequence of the SSU rDNA, ITS 1, 5.8S, ITS 2, and ∼1370 bp at the 5 ′ end of the LSU rDNA was determined in 25 stichotrichs, one oligotrich, and two hypotrichs. Maximum parsimony, neighbor-joining, and quartet-puzzling analyses were used to construct individual phylogenetic trees for SSU rDNA, for LSU rDNA, and ITS 1 + 5.8S + ITS 2, as well as for all these components combined. All trees were similar, with the greatest resolution obtained with the combined components. Phylogenetic relationships were largely consistent with classical taxonomy, with notable disagreements. DNA sequences indicate that Oxytricha granulifera and Oxytricha longa are rather distantly related. The oligotrich, Halteria grandinella, is placed well within the order Stichotrichida. Uroleptus pisces and Uroleptus gallina probably belong to different genera. Holosticha polystylata (family Holostichidae) and Urostyla grandis (family Urostylidae) are rather closely related. These rDNA sequence analyses imply the need for some modifications of classical taxonomic schemes.
ISSN:1055-7903
1095-9513
DOI:10.1016/S1055-7903(03)00097-6