Antioxidant effect of flavonoids on DCF production in HL-60 cells

A fluorometric microplate assay was performed for the detection of respiratory burst activity in a human leukaemia cell line HL‐60 by assessing oxidation of 2′,7′‐dichlorofluorescin diacetate (DCFH‐DA). This method is based on the detection of DCFH oxidation due to the presence of hydrogen peroxide. I...

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Bibliographic Details
Published in:Phytotherapy research 2003-09, Vol.17 (8), p.963-966
Main Authors: Takamatsu, Satoshi, Galal, Ahmed M., Ross, Samir A., Ferreira, Daneel, ElSohly, Mahmoud A., Ibrahim, Abdel-Rahim S., El-Feraly, Farouk S.
Format: Article
Language:English
Subjects:
antioxidants
Antioxidants - administration & dosage
Antioxidants - pharmacology
Antioxidants - therapeutic use
DCFH oxidation
Dose-Response Relationship, Drug
Flavonoids - administration & dosage
Flavonoids - pharmacology
Flavonoids - therapeutic use
Flow Cytometry
Fluoresceins - chemistry
HL-60 cell
HL-60 Cells - drug effects
Humans
Oxidation-Reduction
Phytotherapy
Plants, Medicinal
Respiratory Burst - drug effects
respiratory burst activity
flavonoids
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Summary:A fluorometric microplate assay was performed for the detection of respiratory burst activity in a human leukaemia cell line HL‐60 by assessing oxidation of 2′,7′‐dichlorofluorescin diacetate (DCFH‐DA). This method is based on the detection of DCFH oxidation due to the presence of hydrogen peroxide. In the present study, the antioxidant activity of a number of structurally related flavonoids of plant origin and some of their microbial transformation products (1–18) were evaluated. Copyright © 2003 John Wiley & Sons, Ltd.
ISSN:0951-418X
1099-1573
DOI:10.1002/ptr.1289