A significant reduction of the diaphragm in mdx:MyoD-/-(9th) embryos suggests a role for MyoD in the diaphragm development

To further investigate the role of MyoD during skeletal myogenesis, we backcrossed mdx mutant mice (lacking dystrophin) with MyoD knock-out mice to obtain viable mice with MyoD allele on a pure mdx background. However, after nine generations of backcrossing, it was not possible to obtain a viable md...

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Bibliographic Details
Published in:Developmental biology 2003-09, Vol.261 (2), p.324-336
Main Authors: Inanlou, Mohammad R, Dhillon, Gurmohan S, Belliveau, Anne C, Reid, G Andrew M, Ying, Chuyan, Rudnicki, Michael A, Kablar, Boris
Format: Article
Language:English
Subjects:
Animals
Diaphragm - embryology
DNA-Binding Proteins
Dystrophin - genetics
Dystrophin - metabolism
Extremities - embryology
Genes, Lethal
Mice
Mice, Inbred mdx
Muscle Development - physiology
Muscle Proteins - metabolism
Muscle, Skeletal - embryology
MyoD Protein - genetics
MyoD Protein - metabolism
Myogenic Regulatory Factor 5
Trans-Activators
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Summary:To further investigate the role of MyoD during skeletal myogenesis, we backcrossed mdx mutant mice (lacking dystrophin) with MyoD knock-out mice to obtain viable mice with MyoD allele on a pure mdx background. However, after nine generations of backcrossing, it was not possible to obtain a viable mdx:MyoD-/- phenotype (designated as: mdx:MyoD-/-(9th)). The compound-mutant embryos were examined just before birth. Essentially normal Myf5-dependent and most of the MyoD-dependent musculature was observed. By contrast, the skeletal muscle compartment of the diaphragm was significantly reduced. The mesenchymal compartment of the diaphragm was intact and no herniations were observed. Other examined organs (e.g., liver, kidney, brain, etc.) showed no histological abnormalities. Pulmonary hypoplasia was determined as the cause of neonatal death. Therefore, using a different approach, our new data supplement our previous findings and suggest an essential role for MyoD in development of skeletal muscle of the diaphragm. The failure of mdx:MyoD-/-(9th) diaphragm to develop normally is not caused by a reduced number of satellite cells, but from the inability of stem cells to progress through the myogenic program. Our data also suggest that functions of MyoD and Myf5 (and the respective muscle precursor cell sub-populations) are not entirely redundant by term, as previously suggested, since Myf5 is not capable of fully substituting for MyoD in the diaphragm development.
ISSN:0012-1606
DOI:10.1016/S0012-1606(03)00319-1