Cloning and characterization of Taenia saginata paramyosin cDNA

A lambdaZAP-express cDNA library of Taenia saginata metacestodes was constructed. Antibody screening yielded a clone with an insert of 3,408 bp, an open reading frame of 2,589 bp, a deduced sequence of 863 amino acid and a molecular mass of 98.89 kDa. Alignments of the predicted amino acid sequence...

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Bibliographic Details
Published in:Parasitology research (1987) 2003-09, Vol.91 (1), p.60-67
Main Authors: FERRER, Elizabeth, MOYANO, Eva, PARKHOUSE, E, GARATE, Teresa, BENITEZ, Laura, GONZALEZ, Luis Miguel, BRYCE, Denise, FOSTER-CUEVAS, Mildred, DAVILA, Iris, MILAGROS CORTEZ, Maria, HARRISON, Leslie J. S, MICHAEL, R
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antibodies, Helminth - blood
Biological and medical sciences
Cattle
Cattle Diseases - immunology
Cattle Diseases - parasitology
Cloning, Molecular
Collagen - metabolism
Cysticercosis - immunology
Cysticercosis - parasitology
DNA, Complementary - genetics
Fundamental and applied biological sciences. Psychology
Host parasite relation
pathogenicity
Humans
Invertebrates
Molecular Sequence Data
Nemathelminthia. Plathelmintha
Rabbits
Sequence Alignment
Sequence Analysis, DNA
Swine
Taenia saginata - genetics
Taenia saginata - metabolism
Tropomyosin - chemistry
Tropomyosin - genetics
Tropomyosin - immunology
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Summary:A lambdaZAP-express cDNA library of Taenia saginata metacestodes was constructed. Antibody screening yielded a clone with an insert of 3,408 bp, an open reading frame of 2,589 bp, a deduced sequence of 863 amino acid and a molecular mass of 98.89 kDa. Alignments of the predicted amino acid sequence showed identity with paramyosins from several species: 98.8% with Taenia solium, 96.3% with Echinococcus.granulosus and about 70% with Schistosoma spp. The insert was expressed and purified. A collagen binding assay was performed which showed that T. saginata GST-paramyosin retained this property in a dose-dependent manner. Problems were encountered due to high backgrounds in serological assays in the homologous T. saginata system. However, the recombinant paramyosin was recognized by antibodies present in 31.6% of sera from T. solium seropositive cysticercosis patients and 100% of the sera from acute cysticercosis patients. The immunodominant epitope was the carboxyl-terminal fragment of the molecule.
ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-003-0895-5