Structure of the chicken apo very low density lipoprotein II gene

We describe two cloned genomic DNA fragments, both bearing the entire apo very low density lipoprotein II gene. Electron microscopy and restriction enzyme mapping showed that this gene is split into at least four coding sequences by three or more intervening sequences. A very short exon at the 5...

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Bibliographic Details
Published in:The Journal of biological chemistry 1981-09, Vol.256 (18), p.9668-9671
Main Authors: Meijlink, F C, van het Schip, A D, Arnberg, A C, Wieringa, B, Ab, G, Gruber, M
Format: Article
Language:English
Subjects:
Animals
Apolipoproteins - genetics
Chickens
DNA Restriction Enzymes
DNA, Recombinant - metabolism
Escherichia coli - genetics
Female
Genes
Lipoproteins, VLDL - genetics
Microscopy, Electron
Molecular Weight
Nucleic Acid Hybridization
Plasmids
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Summary:We describe two cloned genomic DNA fragments, both bearing the entire apo very low density lipoprotein II gene. Electron microscopy and restriction enzyme mapping showed that this gene is split into at least four coding sequences by three or more intervening sequences. A very short exon at the 5'-end of the gene is separated by a 1.5-kilobase intron from the second exon, which codes for the AUG initiation codon of the mRNA.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(19)68814-9