Histamine downregulates CD14 expression via H2 receptors on human monocytes

Lipopolysaccharide (LPS) binds to LPS-binding protein (LBP) in plasma and is delivered to the cell surface receptor CD14 on human monocyte. LPS is transferred to the transmembrane signaling receptor toll-like receptor (TLR) 4. In the present study, the effect of histamine on the expression of CD14 o...

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Bibliographic Details
Published in:Clinical immunology (Orlando, Fla.) Fla.), 2003-09, Vol.108 (3), p.274-281
Main Authors: TAKAHASHI, Hideo Kohka, MORICHIKA, Toshihiko, IWAGAKI, Hiromi, TAMURA, Ryuji, KUBO, Shinichiro, YOSHINO, Tadashi, MORI, Shuji, AKAGI, Tadaatsu, TANAKA, Noriaki, NISHIBORI, Masahiro
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cells, Cultured
Dimaprit - pharmacology
Down-Regulation
General aspects
Histamine - pharmacology
Histamine - physiology
Histamine Agonists - pharmacology
Histamine H2 Antagonists - pharmacology
Humans
Infection pathogenesis
Infectious diseases
Leukocytes, Mononuclear
Lipopolysaccharide Receptors - metabolism
Lipopolysaccharides
Medical sciences
Methylhistamines - pharmacology
Monocytes - drug effects
Monocytes - metabolism
Receptors, Histamine H2 - metabolism
Time Factors
Tumor Necrosis Factor-alpha - antagonists & inhibitors
Tumor Necrosis Factor-alpha - biosynthesis
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Summary:Lipopolysaccharide (LPS) binds to LPS-binding protein (LBP) in plasma and is delivered to the cell surface receptor CD14 on human monocyte. LPS is transferred to the transmembrane signaling receptor toll-like receptor (TLR) 4. In the present study, the effect of histamine on the expression of CD14 on human monocytes was investigated. Histamine concentration- and time-dependently decreased the expression of cell surface CD14, whereas histamine did not decrease mRNA for CD14 nor increase soluble CD14 (sCD14). The inhibitory effects of histamine on CD14 expression were antagonized by H2-receptor antagonist, but not by H1 and H3/H4 antagonist. The effects of selective H2-receptor agonists, 4-methylhistamine and dimaprit, on CD14 expression mimicked that of histamine indicating that histamine regulated CD14 expression through the stimulation of H2-receptors. The pretreatment with histamine partially inhibited the LPS-induced TNF-alpha production in human peripheral blood mononuclear cells (PBMC). Such inhibition might be due to the down-regulation of CD14 expression on monocytes by histamine.
ISSN:1521-6616
1521-7035
DOI:10.1016/S1521-6616(03)00140-2