Carbonic anhydrase activity of isolated brush border and basal-lateral membranes of renal tubular cells

Carbonic anhydrase activity was found and characterized in isolated homogeneous preparations of microvilli and basal infoldings of the rat renal tubular cells. This membrane-bound activity was about 3% of the total carbonic anhydrase activity of the renal cortex. Compared to the renal cytoplasmic so...

Full description

Saved in:
Bibliographic Details
Published in:Pflügers Archiv 1977-01, Vol.370 (2), p.121-126
Main Authors: Wistrand, P J, Kinne, R
Format: Article
Language:English
Subjects:
Animals
Basement Membrane - enzymology
Carbonic Anhydrase Inhibitors - pharmacology
Carbonic Anhydrases - metabolism
Ethoxzolamide - pharmacology
Female
Kidney Tubules - enzymology
Kidney Tubules - ultrastructure
Kinetics
Male
Microvilli - enzymology
Rats
Sulfanilamides - pharmacology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c281t-4bac87460ab5dec2655cbe911511388a21123da2d0dae713ac6a4bf0d2f149ac3
cites cdi_FETCH-LOGICAL-c281t-4bac87460ab5dec2655cbe911511388a21123da2d0dae713ac6a4bf0d2f149ac3
container_end_page 126
container_issue 2
container_start_page 121
container_title Pflügers Archiv
container_volume 370
creator Wistrand, P J
Kinne, R
description Carbonic anhydrase activity was found and characterized in isolated homogeneous preparations of microvilli and basal infoldings of the rat renal tubular cells. This membrane-bound activity was about 3% of the total carbonic anhydrase activity of the renal cortex. Compared to the renal cytoplasmic soluble form, the membrane-bound enzymes had the same Km for CO2 and were equally resistant to inhibition by chloride. However, they were less inhibited by different sulfonamides. The implication of the existence of membrane-bound enzymes in the renal tubular cells is discussed.
doi_str_mv 10.1007/BF00581684
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73699662</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>73699662</sourcerecordid><originalsourceid>FETCH-LOGICAL-c281t-4bac87460ab5dec2655cbe911511388a21123da2d0dae713ac6a4bf0d2f149ac3</originalsourceid><addsrcrecordid>eNpFkM1Lw0AQxRfxq1Yvnj3syYMQndlNNpujFqtCwYuew-xHbCRpdDcR-t-b0KKngTe_93g8xi4RbhEgv3tYAmQalU4P2AxTKRIBKA_ZDEBionKlT9lZjJ8AIFItTthxiohQzNjHgoLpNrXltFlvXaDoOdm-_qn7Le8qXseuod47bsIQ19x0wfkwsqNAkZpkegZqeOtbE2jj42QKfjNK_WCGhgK3vmniOTuqqIn-Yn_n7H35-LZ4TlavTy-L-1VihcY-SQ1ZnacKyGTOW6GyzBpfIGaIUmsSiEI6Eg4c-RwlWUWpqcCJCtOCrJyz613uV-i-Bx_7sq3j1GDs1g2xzKUqCqXECN7sQBu6GIOvyq9QtxS2JUI5jVr-jzrCV_vUwbTe_aG7FeUvvxFyjQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>73699662</pqid></control><display><type>article</type><title>Carbonic anhydrase activity of isolated brush border and basal-lateral membranes of renal tubular cells</title><source>Springer Online Journals Archive Complete</source><creator>Wistrand, P J ; Kinne, R</creator><creatorcontrib>Wistrand, P J ; Kinne, R</creatorcontrib><description>Carbonic anhydrase activity was found and characterized in isolated homogeneous preparations of microvilli and basal infoldings of the rat renal tubular cells. This membrane-bound activity was about 3% of the total carbonic anhydrase activity of the renal cortex. Compared to the renal cytoplasmic soluble form, the membrane-bound enzymes had the same Km for CO2 and were equally resistant to inhibition by chloride. However, they were less inhibited by different sulfonamides. The implication of the existence of membrane-bound enzymes in the renal tubular cells is discussed.</description><identifier>ISSN: 0031-6768</identifier><identifier>EISSN: 1432-2013</identifier><identifier>DOI: 10.1007/BF00581684</identifier><identifier>PMID: 411109</identifier><language>eng</language><publisher>Germany</publisher><subject>Animals ; Basement Membrane - enzymology ; Carbonic Anhydrase Inhibitors - pharmacology ; Carbonic Anhydrases - metabolism ; Ethoxzolamide - pharmacology ; Female ; Kidney Tubules - enzymology ; Kidney Tubules - ultrastructure ; Kinetics ; Male ; Microvilli - enzymology ; Rats ; Sulfanilamides - pharmacology</subject><ispartof>Pflügers Archiv, 1977-01, Vol.370 (2), p.121-126</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c281t-4bac87460ab5dec2655cbe911511388a21123da2d0dae713ac6a4bf0d2f149ac3</citedby><cites>FETCH-LOGICAL-c281t-4bac87460ab5dec2655cbe911511388a21123da2d0dae713ac6a4bf0d2f149ac3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/411109$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wistrand, P J</creatorcontrib><creatorcontrib>Kinne, R</creatorcontrib><title>Carbonic anhydrase activity of isolated brush border and basal-lateral membranes of renal tubular cells</title><title>Pflügers Archiv</title><addtitle>Pflugers Arch</addtitle><description>Carbonic anhydrase activity was found and characterized in isolated homogeneous preparations of microvilli and basal infoldings of the rat renal tubular cells. This membrane-bound activity was about 3% of the total carbonic anhydrase activity of the renal cortex. Compared to the renal cytoplasmic soluble form, the membrane-bound enzymes had the same Km for CO2 and were equally resistant to inhibition by chloride. However, they were less inhibited by different sulfonamides. The implication of the existence of membrane-bound enzymes in the renal tubular cells is discussed.</description><subject>Animals</subject><subject>Basement Membrane - enzymology</subject><subject>Carbonic Anhydrase Inhibitors - pharmacology</subject><subject>Carbonic Anhydrases - metabolism</subject><subject>Ethoxzolamide - pharmacology</subject><subject>Female</subject><subject>Kidney Tubules - enzymology</subject><subject>Kidney Tubules - ultrastructure</subject><subject>Kinetics</subject><subject>Male</subject><subject>Microvilli - enzymology</subject><subject>Rats</subject><subject>Sulfanilamides - pharmacology</subject><issn>0031-6768</issn><issn>1432-2013</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1977</creationdate><recordtype>article</recordtype><recordid>eNpFkM1Lw0AQxRfxq1Yvnj3syYMQndlNNpujFqtCwYuew-xHbCRpdDcR-t-b0KKngTe_93g8xi4RbhEgv3tYAmQalU4P2AxTKRIBKA_ZDEBionKlT9lZjJ8AIFItTthxiohQzNjHgoLpNrXltFlvXaDoOdm-_qn7Le8qXseuod47bsIQ19x0wfkwsqNAkZpkegZqeOtbE2jj42QKfjNK_WCGhgK3vmniOTuqqIn-Yn_n7H35-LZ4TlavTy-L-1VihcY-SQ1ZnacKyGTOW6GyzBpfIGaIUmsSiEI6Eg4c-RwlWUWpqcCJCtOCrJyz613uV-i-Bx_7sq3j1GDs1g2xzKUqCqXECN7sQBu6GIOvyq9QtxS2JUI5jVr-jzrCV_vUwbTe_aG7FeUvvxFyjQ</recordid><startdate>19770101</startdate><enddate>19770101</enddate><creator>Wistrand, P J</creator><creator>Kinne, R</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19770101</creationdate><title>Carbonic anhydrase activity of isolated brush border and basal-lateral membranes of renal tubular cells</title><author>Wistrand, P J ; Kinne, R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c281t-4bac87460ab5dec2655cbe911511388a21123da2d0dae713ac6a4bf0d2f149ac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1977</creationdate><topic>Animals</topic><topic>Basement Membrane - enzymology</topic><topic>Carbonic Anhydrase Inhibitors - pharmacology</topic><topic>Carbonic Anhydrases - metabolism</topic><topic>Ethoxzolamide - pharmacology</topic><topic>Female</topic><topic>Kidney Tubules - enzymology</topic><topic>Kidney Tubules - ultrastructure</topic><topic>Kinetics</topic><topic>Male</topic><topic>Microvilli - enzymology</topic><topic>Rats</topic><topic>Sulfanilamides - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wistrand, P J</creatorcontrib><creatorcontrib>Kinne, R</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Pflügers Archiv</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wistrand, P J</au><au>Kinne, R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Carbonic anhydrase activity of isolated brush border and basal-lateral membranes of renal tubular cells</atitle><jtitle>Pflügers Archiv</jtitle><addtitle>Pflugers Arch</addtitle><date>1977-01-01</date><risdate>1977</risdate><volume>370</volume><issue>2</issue><spage>121</spage><epage>126</epage><pages>121-126</pages><issn>0031-6768</issn><eissn>1432-2013</eissn><abstract>Carbonic anhydrase activity was found and characterized in isolated homogeneous preparations of microvilli and basal infoldings of the rat renal tubular cells. This membrane-bound activity was about 3% of the total carbonic anhydrase activity of the renal cortex. Compared to the renal cytoplasmic soluble form, the membrane-bound enzymes had the same Km for CO2 and were equally resistant to inhibition by chloride. However, they were less inhibited by different sulfonamides. The implication of the existence of membrane-bound enzymes in the renal tubular cells is discussed.</abstract><cop>Germany</cop><pmid>411109</pmid><doi>10.1007/BF00581684</doi><tpages>6</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0031-6768
ispartof Pflügers Archiv, 1977-01, Vol.370 (2), p.121-126
issn 0031-6768
1432-2013
language eng
recordid cdi_proquest_miscellaneous_73699662
source Springer Online Journals Archive Complete
subjects Animals
Basement Membrane - enzymology
Carbonic Anhydrase Inhibitors - pharmacology
Carbonic Anhydrases - metabolism
Ethoxzolamide - pharmacology
Female
Kidney Tubules - enzymology
Kidney Tubules - ultrastructure
Kinetics
Male
Microvilli - enzymology
Rats
Sulfanilamides - pharmacology
title Carbonic anhydrase activity of isolated brush border and basal-lateral membranes of renal tubular cells
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-23T21%3A22%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Carbonic%20anhydrase%20activity%20of%20isolated%20brush%20border%20and%20basal-lateral%20membranes%20of%20renal%20tubular%20cells&rft.jtitle=Pfl%C3%BCgers%20Archiv&rft.au=Wistrand,%20P%20J&rft.date=1977-01-01&rft.volume=370&rft.issue=2&rft.spage=121&rft.epage=126&rft.pages=121-126&rft.issn=0031-6768&rft.eissn=1432-2013&rft_id=info:doi/10.1007/BF00581684&rft_dat=%3Cproquest_cross%3E73699662%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c281t-4bac87460ab5dec2655cbe911511388a21123da2d0dae713ac6a4bf0d2f149ac3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=73699662&rft_id=info:pmid/411109&rfr_iscdi=true