Interaction of vanadate with membrane-bound ATPase from Mycobacterium phlei

Vanadate inhibited the formation of proton gradient and membrane potential as well as Ca2+ transport by everted membrane vesicles from Mycobacterium phlei, with half-maximal inhibition occurring at 5 to 14 microM. That this is due to the inhibition of the proton-translocating ATPase was suggested by...

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Bibliographic Details
Published in:The Journal of biological chemistry 1981-12, Vol.256 (23), p.12239-12242
Main Authors: Yoshimura, F, Brodie, A F
Format: Article
Language:English
Subjects:
Adenosine Triphosphatases - metabolism
adenosinetriphosphatase
Cell Membrane - enzymology
Hydrogen-Ion Concentration
Kinetics
Magnesium - pharmacology
Magnesium Chloride
membrane vesicles
Mycobacterium - enzymology
Mycobacterium phlei
Mycobacterium phlei - enzymology
Vanadates
vanadic acid
Vanadium - pharmacology
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Summary:Vanadate inhibited the formation of proton gradient and membrane potential as well as Ca2+ transport by everted membrane vesicles from Mycobacterium phlei, with half-maximal inhibition occurring at 5 to 14 microM. That this is due to the inhibition of the proton-translocating ATPase was suggested by the observation that the inhibition described above occurred only when the processes were driven by the hydrolysis of ATP but not when energized by the oxidation of succinate and NADH. Furthermore, vanadate did indeed inhibit ATP hydrolysis by these membrane vesicles. Although the inhibition of ATP hydrolysis could be demonstrated only in the presence of high concentrations (e.g. 11 mM) of Mg2+, this was presumably due to the fact that we were measuring the sum of ATP hydrolysis by both coupled and partially uncoupled enzymes. This is the first reported effect of vanadate on bacterial proton-translocating ATPase.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)43259-0